Version 1
: Received: 15 November 2023 / Approved: 16 November 2023 / Online: 16 November 2023 (07:50:01 CET)
How to cite:
Unegbu, I.A.; Abdullateef, A.; Mishelia, E.M. Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf. Preprints2023, 2023111052. https://doi.org/10.20944/preprints202311.1052.v1
Unegbu, I.A.; Abdullateef, A.; Mishelia, E.M. Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf. Preprints 2023, 2023111052. https://doi.org/10.20944/preprints202311.1052.v1
Unegbu, I.A.; Abdullateef, A.; Mishelia, E.M. Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf. Preprints2023, 2023111052. https://doi.org/10.20944/preprints202311.1052.v1
APA Style
Unegbu, I.A., Abdullateef, A., & Mishelia, E.M. (2023). Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf. Preprints. https://doi.org/10.20944/preprints202311.1052.v1
Chicago/Turabian Style
Unegbu, I.A., Abdulrahman Abdullateef and Emmanuel Mshelia Mishelia. 2023 "Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf" Preprints. https://doi.org/10.20944/preprints202311.1052.v1
Abstract
Silybum marianum is a therapeutic plant belonging to the family Asteraceae. It contains silymarin, as its principal component, and has been reported to demonstrate hepatoprotective activity. The study was aimed to investigate the phytochemical constituents, synthesis of nanoparticles, antioxidant activity and antimicrobial effects of the leaf extract. Extracts were synthesized from AgNO3 and characterized via FT-IR, U.V and XRD. The antioxidant activity was determined using DPPH, H2O2 and β-carotene assays. Similarly, all extracts expressed significant DPPH inhibition (IC50) of 5.1, 5.0, 4.7, 2.3, 4.3 and 1.1 (ug/mL), H2O2 of 1.3, 2.2, 4.7, 5.6, 4.0 and 1.0 (ug/mL) from the methanol/water extract, nanoparticles, n-Hexane, ETOAC, n-Butanol and Ascorbic acid (standard) respectively. The extract exerted scavenging activity on β-carotene with a significant IC50 inhibition of 1.5, 0.8, 5.2, 0.8, 2.7, 4.8 at 0 hour and 1.5, 0.8, 5.2, 0.8, 2.7 and 4.8 (ug/mL) after 2 hours water bath incubation at 50°C in the order of the extracts above. Additionally, nanoparticles and plant extracts significantly (p<0.0001) inhibited the growth of bacteria with mean zone of inhibition ranging 7-13 mm and 4-11 mm. This study demonstrated that extracts from different solvents of S. marianum possess significant antioxidant activity, as well as antimicrobial effects which are all concentration dependent. Thus, the result suggests that methanolic/water leaf extract of S. marianum could serve as a potential source of antioxidants and can be explored as a therapeutic agent in free radical induced diseases.
Chemistry and Materials Science, Medicinal Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
