Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas

David Azoulay; Tal Tapuchi; Ohad Ronen; Luiza Akria; Hector I Cohen; Celia Surio; Svetlana Rodin Chepa; Moran Zarfati; Galia Stemmer; Elizabeth Eshel; Netanel A Horowitz

doi:10.20944/preprints202311.0952.v2

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preprints.org > medicine and pharmacology > hematology > doi: 10.20944/preprints202311.0952.v2

Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas

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Version 1 : Received: 14 November 2023 / Approved: 14 November 2023 / Online: 15 November 2023 (01:56:10 CET)
Version 2 : Received: 31 December 2023 / Approved: 3 January 2024 / Online: 3 January 2024 (05:31:53 CET)

How to cite: Azoulay, D.; Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Rodin Chepa, S.; Zarfati, M.; Stemmer, G.; Eshel, E.; Horowitz, N.A. Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Preprints 2023, 2023110952. https://doi.org/10.20944/preprints202311.0952.v2 Azoulay, D.; Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Rodin Chepa, S.; Zarfati, M.; Stemmer, G.; Eshel, E.; Horowitz, N.A. Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Preprints 2023, 2023110952. https://doi.org/10.20944/preprints202311.0952.v2

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MDPI and ACS Style

Azoulay, D.; Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Rodin Chepa, S.; Zarfati, M.; Stemmer, G.; Eshel, E.; Horowitz, N.A. Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Preprints 2023, 2023110952. https://doi.org/10.20944/preprints202311.0952.v2

APA Style

Azoulay, D., Tapuchi, T., Ronen, O., Akria, L., Cohen, H.I., Surio, C., Rodin Chepa, S., Zarfati, M., Stemmer, G., Eshel, E., & Horowitz, N.A. (2024). Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Preprints. https://doi.org/10.20944/preprints202311.0952.v2

Chicago/Turabian Style

Azoulay, D., Elizabeth Eshel and Netanel A Horowitz. 2024 "Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas" Preprints. https://doi.org/10.20944/preprints202311.0952.v2

Abstract

Introduction: Flow-cytometry (FC) is a powerful tool that can assist in lymphoma diagnosis in lymph node (LN) specimens. Although lymphoma diagnosis and classification are mainly based on tumor cell characteristics, surrounding cells are less employed in this process.Methods: We retrospectively investigated alterations in the ploidy status, proliferative cell fraction (PF) and the percentages of surrounding immune cells in 62 consecutive LN specimens with B-Cell Non-Hodgkin Lymphoma (B-NHL) that were submitted for FC evaluation between 2019-2022.Results: Compared with indolent B-NHLs, aggressive B-NHLs show increased DNA aneuploidy and PF, increased monocytes, immature-granulocytes, mature granulocytes, CD8⁺ T-cells, Double-Negative-T-cells and Double-Positive-T-cells, and decreased total CD45⁺ cells, total lymphocytes, CD4⁺ T-cells and CD4/CD8 ratio. Receiver operating characteristic analysis determined PF > 6.8% and immature-granulocytes > 0.9% as optimal cutoffs with highest specificity and sensitivity in differentiating aggressive and indolent B-NHLs.Conclusions: These findings further strength the diagnostic value of DNA content analysis by FC and suggest the utilization of tumor surrounding immune cells in NHL diagnosis and classification.

Keywords

Lymph-node (LN); Aggrressive-lymphoma; Indolent-lymphoma; DNA-cell-cycle-indexing; immune-cells; tumor-microenvironment

Subject

Medicine and Pharmacology, Hematology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comments (1)

Comment 1

Received: 3 January 2024
Commenter: David Azoulay
Commenter's Conflict of Interests: Author

Comment: 1. We removed the data of reactive specimens to focus in indolent vs. aggressive specimens. 2.We improved the figure 1 and figure 2 by adding more details and removing information regarding non hematopoietic cells from the illustration. 3. We added an author. 4. We added more references that previously show similar data.

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