PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas
Version 1
: Received: 14 November 2023 / Approved: 14 November 2023 / Online: 15 November 2023 (01:56:10 CET)
Version 2
: Received: 31 December 2023 / Approved: 3 January 2024 / Online: 3 January 2024 (05:31:53 CET)
How to cite:
Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Rodin Chepa, S.; Zarfati, M.; Stemmer, G.; Horowitz, N.A.; Azoulay, D. Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Preprints2023, 2023110952. https://doi.org/10.20944/preprints202311.0952.v1
Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Rodin Chepa, S.; Zarfati, M.; Stemmer, G.; Horowitz, N.A.; Azoulay, D. Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Preprints 2023, 2023110952. https://doi.org/10.20944/preprints202311.0952.v1
Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Rodin Chepa, S.; Zarfati, M.; Stemmer, G.; Horowitz, N.A.; Azoulay, D. Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Preprints2023, 2023110952. https://doi.org/10.20944/preprints202311.0952.v1
APA Style
Tapuchi, T., Ronen, O., Akria, L., Cohen, H.I., Surio, C., Rodin Chepa, S., Zarfati, M., Stemmer, G., Horowitz, N.A., & Azoulay, D. (2023). Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Preprints. https://doi.org/10.20944/preprints202311.0952.v1
Chicago/Turabian Style
Tapuchi, T., Netanel A Horowitz and David Azoulay. 2023 "Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas" Preprints. https://doi.org/10.20944/preprints202311.0952.v1
Abstract
Introduction: Flow-cytometry (FC) is a powerful tool that can assist in lymphoma diagnosis in lymph node (LN) specimens. Although lymphoma diagnosis and classification are mainly based on tumor cell characteristics, surrounding cells are less employed in this process.
Methods: We retrospectively investigated alterations in the ploidy status, proliferative cell fraction (PF) and the percentages of surrounding immune cells in 62 consecutive LN specimens with B-Cell Non-Hodgkin Lymphoma (B-NHL) and 26 gender and age mached reactive LN (RLN) specimens that were submitted for FC evaluation between 2019-2022.
Results: B-NHL samples showed increased rate of DNA aneuploidy and PF, increased CD45+ cells, lymphocytes and B-cells, and decreased T-cells compared to RLN. Compared with indolent B-NHLs, aggressive B-NHLs show increased DNA aneuploidy and PF, increased monocytes, immature-granulocytes, mature granulocytes, CD8+ T-cells, Double-Negative-T-cells and Double-Positive-T-cells, and decreased CD45+ cells, lymphocytes, CD4+ T-cells and CD4/CD8 ratio. Receiver operating characteristic analysis determined integration of PF > 6.8%, immature-granulocytes > 0.9% and Double-Negative-T-cells >3.1% as optimal cutoffs with highest specificity and sensitivity in differentiating aggressive and indolent B-NHLs.
Conclusions: These findings further strengh the diagnostic value of DNA content analysis by FC and suggest the utilization of tumor surrounding immune cells in NHL diagnosis and classification.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
