Version 1
: Received: 8 November 2023 / Approved: 8 November 2023 / Online: 8 November 2023 (15:32:17 CET)
How to cite:
Tiwutanon, P.; Wongthet, N.; Vajrodaya, S.; Kraichak, E. Chemical Profiles and Chemometric Analysis of Selected Leucobryum Species (Bryophyta, Leucobryaceae) from Thailand. Preprints2023, 2023110569. https://doi.org/10.20944/preprints202311.0569.v1
Tiwutanon, P.; Wongthet, N.; Vajrodaya, S.; Kraichak, E. Chemical Profiles and Chemometric Analysis of Selected Leucobryum Species (Bryophyta, Leucobryaceae) from Thailand. Preprints 2023, 2023110569. https://doi.org/10.20944/preprints202311.0569.v1
Tiwutanon, P.; Wongthet, N.; Vajrodaya, S.; Kraichak, E. Chemical Profiles and Chemometric Analysis of Selected Leucobryum Species (Bryophyta, Leucobryaceae) from Thailand. Preprints2023, 2023110569. https://doi.org/10.20944/preprints202311.0569.v1
APA Style
Tiwutanon, P., Wongthet, N., Vajrodaya, S., & Kraichak, E. (2023). Chemical Profiles and Chemometric Analysis of Selected Leucobryum Species (Bryophyta, Leucobryaceae) from Thailand. Preprints. https://doi.org/10.20944/preprints202311.0569.v1
Chicago/Turabian Style
Tiwutanon, P., Srunya Vajrodaya and Ekaphan Kraichak. 2023 "Chemical Profiles and Chemometric Analysis of Selected Leucobryum Species (Bryophyta, Leucobryaceae) from Thailand" Preprints. https://doi.org/10.20944/preprints202311.0569.v1
Abstract
Leucobryum is a moss genus that exhibits various bioactivities. However, the identification of Leucobryum species with morphology alone remains difficult. Nowadays, chemical profile analysis provides additional tools for plant classification, especially in medicinal plants. This method uses chemical similarities to identify the differences among some plants, especially for the different varieties of the same species. The objective of this study was to obtain the chemical profiles and use chemometrics to identify selected Leucobryum species found in Thailand. Lipophilic extracts from 18 samples of five taxa were analyzed with TLC and HPLC. Cluster analysis and PCA were used to investigate the similarities of the chemical profiles. PERMANOVA was performed to determine the difference among taxa. The results showed that the TLC profile could separate only the genus of the sample, while HPLC chromatograms showed separation among the Leucobryum taxa and consistent patterns within the same species. In addition, the chemical profiles of Leucobryum species were separated at the species level in PCA. The PERMANOVA showed significantly different profiles among the species (P = 0.002). Therefore, this work illustrated the potential of chemometrics as supporting evidence for species delimitation in the moss genus Leucobryum.
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
