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Immunoblotting Identification of Diagnostic Antigens of Paragonimus westermani Type 1 for Detection of Human Pulmonary Paragonimiasis in North East India
Devi, K.R.; Deka, A.; Mukherjee, D.; Kaur, H.; Narain, K. Immunoblotting Identification of Diagnostic Antigens of Paragonimus westermani Type 1 for the Detection of Human Pulmonary Paragonimiasis in North East India. Trop. Med. Infect. Dis.2024, 9, 6.
Devi, K.R.; Deka, A.; Mukherjee, D.; Kaur, H.; Narain, K. Immunoblotting Identification of Diagnostic Antigens of Paragonimus westermani Type 1 for the Detection of Human Pulmonary Paragonimiasis in North East India. Trop. Med. Infect. Dis. 2024, 9, 6.
Devi, K.R.; Deka, A.; Mukherjee, D.; Kaur, H.; Narain, K. Immunoblotting Identification of Diagnostic Antigens of Paragonimus westermani Type 1 for the Detection of Human Pulmonary Paragonimiasis in North East India. Trop. Med. Infect. Dis.2024, 9, 6.
Devi, K.R.; Deka, A.; Mukherjee, D.; Kaur, H.; Narain, K. Immunoblotting Identification of Diagnostic Antigens of Paragonimus westermani Type 1 for the Detection of Human Pulmonary Paragonimiasis in North East India. Trop. Med. Infect. Dis. 2024, 9, 6.
Abstract
Human pulmonary paragonimiasis, an emerging concern in North East India, frequently masquerades as pulmonary tuberculosis due to clinical and radiological similarities, leading to diagnostic challenges. This research was aimed to harness the immunoblotting technique to discern immunodiagnostic protein antigens from both adult worm and excretory-secretory (ES) extracts of the prevalent Paragonimus westermani type 1 in Arunachal Pradesh, North East India. We studied the time kinetics of immunoreactive patterns in relation to the duration of infection in rodent models. Immunoblot analyses were also conducted using sera from ELISA positive patients confirmed with paragonimiasis, facilitating the selection of antigenic extracts with diagnostic potential. Further, ES proteins antigens were subjected to 2D- immunoblot analysis and immunoreactive protein spots identified by MALDI-TOF MS. Immunoreactivity patterns of ES antigens with sera of paragonimiasis positive patients were detailed, and specific immunoreactive protein antigens were pinpointed using peptide mass fingerprinting (MALDI-TOF). This work underscores the enhanced diagnostic accuracy when combining ELISA with immunoblotting for pulmonary paragonimiasis in regions like North East India, marked by co-existing helminth infections.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
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