Cardoso, I.R.; De Lima, C.S.; Dos Reis, R.B.; Pinto, A.C.A.; Pissinatti, T.; Kugelmeier, T.; Neto, S.F.D.C.; Da Silva, F.A.; Santos, H.L.C. Occurrence of Free-Living Amoebae in Non-Human Primate Gut. Preprints2023, 2023102110. https://doi.org/10.20944/preprints202310.2110.v1
APA Style
Cardoso, I.R., De Lima, C.S., Dos Reis, R.B., Pinto, A.C.A., Pissinatti, T., Kugelmeier, T., Neto, S.F.D.C., Da Silva, F.A., & Santos, H.L.C. (2023). Occurrence of Free-Living Amoebae in Non-Human Primate Gut. Preprints. https://doi.org/10.20944/preprints202310.2110.v1
Chicago/Turabian Style
Cardoso, I.R., Fabio Alves Da Silva and Helena Lúcia Carneiro Santos. 2023 "Occurrence of Free-Living Amoebae in Non-Human Primate Gut" Preprints. https://doi.org/10.20944/preprints202310.2110.v1
Abstract
. Gut microbiome reflects healthy and predicts possible disease in hosts. A holistic view of this community is needed, focused on identification species, and on the dissection of how species interact with their host and with each other, regardless of whether their presence is beneficial, inconsequential, or detrimental. Distribution of gut-associated eukaryotes within and across NHPs is likely driven by host behavior and ecology. To ascertain the existence of free-living amoebae (FLA) in the gut of wild and captive non-human primates (NHPs), 01 sample stools were collected and submitted to culture-dependent and microscopy examination and DNA sequencing. FLAs were detected in 45.4% (46/101) of fecal samples analysed and their morphological characteristics matching those of Acanthamoeba spp, Vermamoeba spp, and fan-shaped amoebae of the family Vannellidae. Sequence analysis of the PCR- products revealed that the suspected amoebae are highly homologous (99% identity and 100% query coverage) with Acanthamoeba genotype T4 and Vermamoeba vermiformis amoebae. The results showed a great diversity of FLAs in the NHP microbiome, which may pose a potential risk to the health of NHPs. To the best of our knowledge, this is the first report the presence of FLAs in NHPs naturally infected. However, it is not known whether gut-borne FLA are exploiting a viable ecological niche, or whether they are simply transient residents in the gut.
Copyright:
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