Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells

Polina Petkova-Kirova
† ORCID logo ,
Nicoletta Murciano
† ,
Julia Jansen
† ,
Greta Simionato
† ,
Giulia Iacono
† ,
Maria Giustina Rotordam
,
Thomas John
,
Min Qiao
ORCID logo ,
Laura Hertz
,
Arjan J. Hoogendijk
,
Nadine Becker
,
Christian Wagner
,
Marieke von Lindern
ORCID logo ,
Stephane Egee
ORCID logo ,
Emile Van den Akker
ORCID logo ,
Lars Kaestner
* ORCID logo
†
Equal contribution.
Version 1 : Received: 16 October 2023 / Approved: 17 October 2023 / Online: 17 October 2023 (08:00:05 CEST)

A peer-reviewed article of this Preprint also exists.

Petkova-Kirova, P.; Murciano, N.; Iacono, G.; Jansen, J.; Simionato, G.; Qiao, M.; Van der Zwaan, C.; Rotordam, M.G.; John, T.; Hertz, L.; Hoogendijk, A.J.; Becker, N.; Wagner, C.; Von Lindern, M.; Egee, S.; Van den Akker, E.; Kaestner, L. The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells. Int. J. Mol. Sci. 2024, 25, 1416. Petkova-Kirova, P.; Murciano, N.; Iacono, G.; Jansen, J.; Simionato, G.; Qiao, M.; Van der Zwaan, C.; Rotordam, M.G.; John, T.; Hertz, L.; Hoogendijk, A.J.; Becker, N.; Wagner, C.; Von Lindern, M.; Egee, S.; Van den Akker, E.; Kaestner, L. The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells. Int. J. Mol. Sci. 2024, 25, 1416.

Abstract

(1) Background: The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in the red blood cell (RBC) membrane. Nevertheless, the quantitative electrophysiological behavior of RBCs and its heterogeneity are still not completely understood.; (2) Methods: Here we use state-of-the-art biochemical methods to probe for the abundance of the channels in RBCs. Furthermore, we utilize automated patch-clamp, based on planar chips, to compare the activity of the two channels in reticulocytes and mature RBCs. Besides this characterization, we performed membrane potential measurements to demonstrate the effect of channel activity and interplay on the RBC properties.; (3) Results: Both, Gárdos channel and Piezo1, albeit their average copy number of activatable channels per cell is in the single digit range, can be detected by transcriptome analysis of reticulocytes. Proteomics analysis of RBCs could only detect Piezo1 but not the Gárdos channel. Furthermore, they can be reliably measured in the whole-cell configuration of the patch-clamp method. While for the Gárdos channel the activity is higher in reticulocytes compared to mature RBCs, for Piezo1 the tendency is the opposite. While the interplay between Piezo1 and Gárdos channel cannot be followed using the patch-clamp measurements, it could be proved based on membrane potential measurements in populations of intact RBCs.; (4) Conclusions: We discuss the Gárdos channel and Piezo1 abundance, interdependencies and interactions in the context of their proposed physiological and pathophysiological functions, which are the passing of small constrictions, e.g., in the spleen, and their active participation in blood clot formation and thrombosis.

Keywords

erythrocytes; reticulocytes; patch-clamp; membrane potential; NS309; Yoda1; TRAM34; calcium

Subject

Biology and Life Sciences, Biophysics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.