Danilova, O.V.; Oshkin, I.Y.; Belova, S.E.; Miroshnikov, K.K.; Ivanova, A.A.; Dedysh, S.N. One Step Closer to Enigmatic USCα Methanotrophs: Isolation of a Methylocapsa-like Bacterium from a Subarctic Soil. Microorganisms2023, 11, 2800.
Danilova, O.V.; Oshkin, I.Y.; Belova, S.E.; Miroshnikov, K.K.; Ivanova, A.A.; Dedysh, S.N. One Step Closer to Enigmatic USCα Methanotrophs: Isolation of a Methylocapsa-like Bacterium from a Subarctic Soil. Microorganisms 2023, 11, 2800.
Danilova, O.V.; Oshkin, I.Y.; Belova, S.E.; Miroshnikov, K.K.; Ivanova, A.A.; Dedysh, S.N. One Step Closer to Enigmatic USCα Methanotrophs: Isolation of a Methylocapsa-like Bacterium from a Subarctic Soil. Microorganisms2023, 11, 2800.
Danilova, O.V.; Oshkin, I.Y.; Belova, S.E.; Miroshnikov, K.K.; Ivanova, A.A.; Dedysh, S.N. One Step Closer to Enigmatic USCα Methanotrophs: Isolation of a Methylocapsa-like Bacterium from a Subarctic Soil. Microorganisms 2023, 11, 2800.
Abstract
Scavenging of atmospheric trace gases has been recognized as one of the lifestyle-defining capabilities of microorganisms in terrestrial polar ecosystems. Several metagenome-assembled genomes of as-yet-uncultivated methanotrophic bacteria, which consume atmospheric CH4 in these ecosystems, have been retrieved in cultivation-independent studies. In this study, we isolated and characterized a representative of these methanotrophs, strain D3K7, from a subarctic soil of Northern Russia. Strain D3K7 grows on methane and methanol in a wide range of temperatures, between 5 and 30°C. Weak growth was also observed on acetate. The presence of acetate in the culture medium stimulated growth at low CH4 concentrations (~100 p.p.m.v.). The finished genome sequence of strain D3K7 is 4.15 Mb in size and contains about 3700 protein-encoding genes. According to the result of phylogenomic analysis, this bacterium forms a common clade with metagenome-assembled genomes obtained from the active layer of a permafrost thaw gradient in Stordalen Mire, Abisco, Sweeden, and the mineral cryosol at Axel Heiberg Island in the Canadian high Arctic. This clade occupies a phylogenetic position in between characterized Methylocapsa methanotrophs and representatives of the as-yet-uncultivated Upland Soil Cluster Alpha (USCα). As shown by the global distribution analysis, D3K7-like methanotrophs colonize mostly northern terrestrial ecosystems, such as polar deserts, subarctic soils, permafrost environments, but also occur in boreal peatlands or high-latitude habitats.
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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