Article
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The Immunoprotective Analysis of ROP 27 Protein in Eimeria tenella
Version 1
: Received: 12 October 2023 / Approved: 13 October 2023 / Online: 13 October 2023 (07:49:42 CEST)
A peer-reviewed article of this Preprint also exists.
Li, M.; Lv, X.; Zheng, M.; Wei, Y. The Immunoprotective Effect of ROP27 Protein of Eimeria tenella. Animals 2023, 13, 3500. Li, M.; Lv, X.; Zheng, M.; Wei, Y. The Immunoprotective Effect of ROP27 Protein of Eimeria tenella. Animals 2023, 13, 3500.
Abstract
Eimeria tenella rhoptry protein has the properties of a protective antigen. EtROP27 is a pathogenic related gene detected by transcriptome, but its expression pattern, immunogenicity and potency have been unknown. Therefore, a gene segment of EtROP27 was amplified and transplanted to pET28a prokaryotic vector for recombinant protein expression and purified to generate polyclonal antibody. Then, RT-PCR, Western blotting were performed to know the expression pattern of EtROP27. Subsequently, animal experiments were conducted to evaluate the immunoprotective effect of the recombinant protein using different immunizing doses (50, 100, 150 μg). The results showed that the expression of EtROP27 gradually increased with the prolongation of infection time, reaching the highest level after 96 hours and then decreased. And EtROP27 was a natural antigen of coccidia which can stimulate the body to produce high levels of IgY. As recombinant protein vaccines, the immune protection evaluation tests of EtROP27 showed that the average weight gain rate of the immune challenge groups were significantly higher than that of the infection control group. The average lesion scores were significantly lower than that of the control group. The oocyst excretion decreased by 81.25%, 86.21%, 80.01% and the anticoccidial index were 159.45, 171.47, 166.75. EtROP27 is a promising candidate antigen gene for development of coccidiosis vaccine.
Keywords
Eimeria tenella; recombinant EtROP27 protein; expression changes; immunoprotective
Subject
Biology and Life Sciences, Parasitology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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