Version 1
: Received: 25 September 2023 / Approved: 26 September 2023 / Online: 28 September 2023 (13:01:45 CEST)
Version 2
: Received: 13 March 2024 / Approved: 14 March 2024 / Online: 14 March 2024 (11:12:37 CET)
How to cite:
Finding, E.J.; Faulkner, A.; Wheeler-Jones, C.P. Equine Endothelial Cells Show Pro-angiogenic Behaviours in Response to FGF2 but Not VEGF-A. Preprints2023, 2023092006. https://doi.org/10.20944/preprints202309.2006.v2
Finding, E.J.; Faulkner, A.; Wheeler-Jones, C.P. Equine Endothelial Cells Show Pro-angiogenic Behaviours in Response to FGF2 but Not VEGF-A. Preprints 2023, 2023092006. https://doi.org/10.20944/preprints202309.2006.v2
Finding, E.J.; Faulkner, A.; Wheeler-Jones, C.P. Equine Endothelial Cells Show Pro-angiogenic Behaviours in Response to FGF2 but Not VEGF-A. Preprints2023, 2023092006. https://doi.org/10.20944/preprints202309.2006.v2
APA Style
Finding, E.J., Faulkner, A., & Wheeler-Jones, C.P. (2024). Equine Endothelial Cells Show Pro-angiogenic Behaviours in Response to FGF2 but Not VEGF-A. Preprints. https://doi.org/10.20944/preprints202309.2006.v2
Chicago/Turabian Style
Finding, E.J., Ashton Faulkner and Caroline PD Wheeler-Jones. 2024 "Equine Endothelial Cells Show Pro-angiogenic Behaviours in Response to FGF2 but Not VEGF-A" Preprints. https://doi.org/10.20944/preprints202309.2006.v2
Abstract
Understanding the factors which control endothelial cell (EC) function and angiogenesis is crucial for developing the horse as a disease model, but equine ECs remain poorly studied. In this study we have optimised methods for the isolation and culture of equine aortic endothelial cells (EA-oECs) and characterised their angiogenic functions in vitro. Mechanical dissociation, followed by magnetic purification using an anti-VE-cadherin antibody, resulted in EC-enriched cultures suitable for further study. Fibroblast growth factor 2 (FGF2) increased EAoEC proliferation rate and stimulated scratch wound closure and tube formation by EAoECs on extracellular matrix. Pharmacological inhibitors of FGFR1 (SU5402) or MEK (PD184352) blocked FGF2-induced ERK1/2 phosphorylation and functional responses, suggesting that these are dependent on FGFR1/MEK-ERK signalling. In marked contrast, VEGF-A had no effect on EAoEC proliferation, migration or tubulogenesis and did not promote ERK1/2 phosphorylation, indicating a lack of sensitivity to this classical pro-angiogenic growth factor. Gene expression analysis showed that, unlike human ECs, FGFR1 is expressed by EAoECs at a much higher level than both VEGFR1 and VEGFR2. These results suggest a predominant role for FGF2 versus VEGF-A in controlling the angiogenic functions of equine ECs. Collectively, our novel data provide a sound basis for stud-ying angiogenic processes in the horse and lay the foundations for comparative studies of EC biology in horses versus humans.
Keywords
equine; endothelial; angiogenesis; comparative
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
