Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In vitro Efficacy of Isobutyl-Cyanoacrylate Nanoparticles Against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout (Onchorhyncus mykiss)

Gabriel Mawuko Ahiable
ORCID logo ,
Kouki Matsunaga
,
Mao Hokin
,
Kazuhiro Iida
,
Fumiaki Befu
,
Syun-Ichirou Oshima
*
Version 1 : Received: 16 September 2023 / Approved: 18 September 2023 / Online: 18 September 2023 (08:45:17 CEST)

A peer-reviewed article of this Preprint also exists.

Ahiable, M.G.; Matsunaga, K.; Hokin, M.; Iida, K.; Befu, F.; Oshima, S.-I. In Vitro Efficacy of Isobutyl Cyanoacrylate Nanoparticles against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout (Oncorhynchus mykiss). Microorganisms 2023, 11, 2877. Ahiable, M.G.; Matsunaga, K.; Hokin, M.; Iida, K.; Befu, F.; Oshima, S.-I. In Vitro Efficacy of Isobutyl Cyanoacrylate Nanoparticles against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout (Oncorhynchus mykiss). Microorganisms 2023, 11, 2877.

Abstract

The upsurge in and havoc being wrecked by antibiotic resistant bacteria makes urgent the need for efficacious alternatives to antibiotics. This study assessed the antibacterial efficacy of two isobutyl-cyanoacrylate nanoparticles (iBCA-NPs), D6O and NP30, against major bacterial pathogens of fish. In vivo tests on rainbow trout were preceded by tests of in vitro minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). NP30 exhibited higher efficacy, but both iBCA-NPs demonstrated dose-dependent and species-specific in vitro antibacterial properties against the bacterial isolates. Generally, Gram-negative bacteria were more resistant to the iBCA-NPs. Streptococcus iniae, Tenacibaculum maritimum, and Photobacterium damselae were particularly sensitive to both iBCA-NPs. Orally administered to rainbow trout, the iBCA-NPs produced relative gain rate and survival rates comparable to the control (P > 0.05). Condition factor, hepatosomatic and viscerosomatic indices of fish were indifferentiable (P > 0.05) between the iBCA-NP groups and the control. The iBCA-NPs caused no alteration in stress, oxidative stress (superoxide dismutase, SOD), plasma complement titre, and lysozyme activity. The results of this study suggest that D60 and NP30 may contribute to reducing the amount of antibiotics and chemotherapeutic agents being used in aquaculture.

Keywords

antibacterial efficacy; isobutyl-cyanoacrylate nanoparticles; rainbow trout; safety

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.