Short-Term Nitrous Oxide Emissions from Cattle Slurry for Silage Maize: Effects of Placement and the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

1. Introduction

In agroecosystems, nitrogen (N) is an essential nutrient and a key limiting factor for the growth and develop¬ment of crops (Spiertz, 2010). Animal manure has been used as a source of N for centuries. Generally, the application of organic fertilizers increases the risk for environmental losses via ammonia volatilization, leaching, or emissions of other N gases including nitrous oxide (N₂O) emissions. Intensive dairy production is dominated by liquid manure management (Leip et al., 2009), and field application of liquid manure (slurry) involves risks for environmental impacts via ammonia volatilization, leaching, and N₂O emissions. Ammonia volatilization from slurry is effectively reduced by injection or immediate incorporation (Webb et al., 2010; Hafner et al., 2018). When cattle slurry is applied before seeding of spring crops such as maize, nitrate (NO₃^-) leaching is a risk depending mainly on soil type and rainfall during spring. For example, in a lysimeter study with maize on a coarse sandy soil, Nair et al. (2020) observed a 3- to 4-fold increase in NO₃^- leaching due to cattle slurry application when simulating high rainfall during spring. Nitrous oxide has a high global warming potential (GWP) of 265 with a 100-year time horizon and plays a central role in the depletion of stratospheric ozone (Ravishankara et al., 2009; Myhre et al., 2013). Both nitrification and denitrification can be potential sources of N₂O from agricultural soil, however, studies under controlled laboratory conditions (e.g., Zhu et al., 2013; Duan et al., 2017) as well as field conditions (Ibraim et al., 2020) have found that denitrification and nitrifier denitrification are the main sources of N₂O emissions.

Following field application, manure-saturated soil volumes result in the development of organic hotspots supporting nitrification and denitrification for several weeks (Petersen et al., 1992; Nielsen and Revsbech, 1994). With a high content of ammoniacal N, water, and easily degradable carbon (C), manure hotspots can become anoxic because of diffusional constraints and elevated oxygen (O₂) consumption rates for microbial respiration (Weir et al., 1993), and in anoxic volumes electrons then are available for denitrification (Akiyama et al., 2010; VanderZaag et al., 2011).

The conversion of ammonia to nitrite and nitrate in the soil around manure hotspots is often the limiting factor in coupled nitrification-denitrification. Preventing nitrification using a nitrification inhibitor (Zerulla et al., 2001) can be an effective strategy to mitigate N₂O emissions from manure-treated soil (Peixoto and Petersen, 2023). Nitrification inhibitors can desynchronize C and N turnover after slurry application by limiting the availability of NO3- as electron acceptor for denitrification. Synthetic nitrification inhibitors have been widely shown to suppress the activity of ammonia-oxidizing bacteria by inhibition of ammonia-monooxygenase (AMO), the enzyme responsible for the first step in the oxidation of ammonium (NH₄⁺) to nitrite (NO₂⁻), thereby reducing the nitrification rate and the risk for subsequent losses through NO₃⁻ leaching or denitrification and N₂O emissions (Ruser and Schulz, 2015; Beeckman et al., 2018; Zhou et al., 2020). Nitrification inhibitors are sensitive to temperature, and at 20C the inhibitory effect is lost within 5-6 weeks (Subbarao et al., 2006).

Silage maize as feed for dairy cattle is a main crop in Denmark, especially on sandy soils, and approximately 7% of the total agricultural area is under maize (Danmarks Statistik, 2020). Cattle slurry is being widely used as a fertilizer for maize. Traditionally cattle slurry is injected before soil ploughing and seed bed preparation to reduce N losses. Another strategy to decrease environmental N losses, including N₂O emissions, after slurry application is to improve the ability of living roots to take up nutrients such as N. This can be achieved by placing the slurry close to crop seeds for better root access (Pedersen et al., 2022). The infiltration of slurry liquid into the soil is impeded by sealing effects (Miller et al., 1985) and water retention by organic dry matter in the manure (Petersen et al., 2003). Therefore, after placement of slurry under the seed, suspended and dissolved slurry organic matter gets lodged inside pores of the soil surrounding the slurry-saturated soil (Barrington and Madramootoo, 1989), increasing the retention of N in the root zone. Maize seedlings prefer uptake of NH₄⁺ over NO₃^- (Zhang et al., 2019). In addition, placement of slurry close to maize seeds can also increase the initial phosphorus uptake of the young plants (Pedersen et al., 2022).

The main objective of this study was to investigate effects of cattle slurry application method (traditional direct injection and ploughing vs. placement as a broad band under maize), and use of the nitrification inhibitor Vizura® with the active compound 3,4-dimethylpyrazole phosphate (DMPP), on soil mineral nitrogen and N₂O emission dynamics during the initial growth phase with signficant soil N dynamics. It was hypothesized that DMPP would reduce N₂O emissions independent of slurry distribution, and that placement of slurry would reduce N₂O emissions compared to non-placed slurry by enhancing the plant removal of soil mineral N from the soil solution.

2. Materials and Methods

2.1. Field experiment

The field experiment was conducted in 2020 at Foulumgaard (56°49′ N; 9°56′ E); an experimental farm at the Viborg campus of Aarhus University; Denmark. The 3 × 18 m2 experimental plots; established to evaluate N and P utilization of maize (Pedersen et al.; 2022); were organized as a randomized block design with three replications (Table 2; except that non-fertilized sampling positions had to be placed in a separate plot. The soil is a sandy loam based on ground morainic deposits from the last glaciation classified as a Mollic Luvisol according to the WRB (FAO) system (Krogh and Greve; 1999). Selected soil properties are shown in Table 1. Figure 1 (in results section) shows temperature and rain events during experimental period

The cattle slurry was obtained from a local farm. It contained 5.6 % dry matter, 3.4 kg total N Mg^-1 and 1.9 kg NH₄⁺-N Mg^-1. All fertilized plots received cattle slurry at a rate of 110 kg NH₄⁺-N ha^-1, equivalent to 57 Mg ha^-1. Where required, the nitrification inhibitor Vizura® (BASF, 97 Ludwigshafen, Germany) with the active compound 3,4-dimethylpyrazole phosphate (DMPP) was admixed in the slurry tanker at a rate corresponding to 2 L ha^-1 before field application. In treatments without placement (called non-placed slurry), cattle slurry was injected using a traditional closed-slot injector with narrow tines at 10 cm depth at a band width of 25 cm, and this was immediately followed by ploughing (0-25 cm depth) before seeding. In treatments with slurry placement, cattle slurry was injected into ploughed soil in a broad band (about 30 cm broad) with the upper edge of the slurry band at 10 cm soil depth. Here, slurry injection was performed with a 26 cm wide goosefoot tine and a roller to ensure an accurate injection depth. The distance between tines was 75 cm, corresponding to the distance between maize rows in this trial. Maize (Zea mays) was sown at 5 cm depth immediately over the injection bands with placed slurry. All fertilized treatments also received 27 kg N ha^-1 in ammonium sulphate as a starter fertilizer placed 5 cm beside the seeds (Pedersen et al., 2022).

2.2. N₂O measurements

Monitoring of N₂O took place between 29 April and 22 June 2020 with a total of 12 sampling days, i.e., three times during the first week and then at weekly intervals. Flux measurements were conducted using 37 cm × 27 cm × 22 cm static chambers placed over 35 cm × 25 cm × 15 cm support frames of stainless steel that were permanently installed within or between maize crop rows. The chambers were made of PVC and covered with an outer layer of Aluthermo Quattro (Adflexion Aps, Odense, Denmark) for insulation and reflection of solar radiation; they had a butyl rubber septum for gas sampling and a rubber seal at the bottom; together with an elastic strap fixed to the ground on either side of the chamber, this ensured a tight seal during measurements. The support frames were placed with the long side perpendicular to crop rows, i.e., row and inter-row positions together covered 70 of the 75 cm row and inter-row area. The support frames were installed immediately after slurry application, and only temporarily removed for seeding after 5-6 days.

Gas sampling was generally completed between mid-morning and noon. Ten ml gas samples were collected using a 10 mL plastic syringe with hypodermic needle immediately after chamber deployment, and at three additional time points; each time the syringe was purged several times with headspace air before withdrawing the gas sample. Gas samples were stored in pre-evacuated 6 mL exetainer vials (Labco Ltd., Ceredigion, UK) until analysis for N₂O using an Agilent 7890 (Agilent, Nærum, Denmark) gas chromatograph configured as previously described by Petersen et al. (2012).

2.3. Soil sampling

Soil samples were collected in the five main treatments (Table 2) on the same days as gas sampling. Six individual subsamples were randomly taken from each treatment and block using an auger (2 cm diameter, 0–20 cm depth), and from each plot were pooled and transferred to zip-lock plastic bags and stored at 2 °C until analyzed. Soil samples were mixed and sieved (< 6 mm mesh size), and subsamples extracted for analysis of NH₄⁺-N and NO₃^--N, within two days of soil sampling. Approximately 10 g of soil was extracted in 40 ml of 1 M KCl for 30 min (end-over-end) and then allowed to settle. The supernatant was filtered (1.6 μm glass microfiber filters; VWR, Sweden) and frozen at −20 °C for later analysis on a AA500 Autoanalyzer (SEAL analytical GmbH, Norderstedt, Germany). Gravimetric soil water content was determined by drying c. 10 g of soil for 24 h at 105 °C.

2.4. Distribution of mineral N after placement

In a separate activity, additional soil samples were taken on day 2, 10 and 18 of the monitoring periods to map the horizontal and vertical distribution of mineral N after placement of cattle slurry with or without Vizura. Soil samples (2 cm diam., 0-20 cm depth) were taken at the center of the slurry band, and at 10 and 20 cm distance on either side; these samples were taken in three different positions along the length of the slurry band 10 cm apart. Each soil core was sub-divided into three depth intervals (0-5, 5-10, and 10-20 cm depth), and subsamples from each distance and depth were then pooled for analysis, i.e., for each depth three subsamples were pooled in the in-row position, and six subsamples per depth at 10 and 20 cm distance from the crop row. Hence, there were in total nine pooled samples from each of the two treatments. With three replicate field plots and three sampling days, there were162 individual soil samples for analysis of moisture content and mineral N (NH₄⁺-N and NO₃^--N) as explained above.

2.5. Data analyses

Nitrous oxide fluxes were calculated by the HMR package available as a package in R-programming language (Pedersen et al., 2010). Parameter settings were as explained in Meng et al. (2023). Table 3 shows an overview of treatments and chamber placements.

Statistical analyses of the data were performed using R programming language version 4.1.2. Temporal N₂O fluxes and cumulative N₂O were analyzed for the inter-row (IR) and within-row (Row) positions separately, as well as together, and in both cases excluding control treatments (0N). Mineral N data for all main treatments were analyzed as well. Main and interacting effects of slurry application method and use of Vizura were investigated with block as a random factor in the analyses. All data were analyzed with the linear mixed effect (lme) function of the nlme package using the restricted maximum likelihood (REML) method. Model assumptions, i.e., normality and homogeneity of variance, were assessed using diagnostic plots of residuals. In order to satisfy model assumptions, the daily and cumulative N₂O emissions and mineral N (from both main treatments and the separate samplings around placed slurry) were log-transformed. For time series of N₂O emissions and mineral N data, auto-correlation between sampling positions were accounted with the corAR1 function. Pairwise comparisons between treatments were performed using the estimated marginal means (emmeans) function. The p-values were adjusted by Tukey's HSD method, and the hypothesis rejection threshold was 0.05.

3. Results

3.1. Environmental conditions

Figure 1 shows daily average air temperature and precipitation during the monitoring period, and the daily mean air temperature ranged from 5.5 to 18.9 °C. Daily rainfall ranged from 0 to a maximum of 17 mm, the latter occurring one day after placement of slurry, and the cumulative rainfall was 97 mm during the period of measurement.

3.2. Soil moisture and mineral N dynamics

Soil gravimetric water content on the sampling days varied between 18 and 21 % during the experimental period; assuming a bulk density of 1.37 g cm^-3, the bulk denisty recorded in a neighboring field under similar management (ploughed soil, crop residues removed). The calculated range in water content corresponded to 48-56 % water-filled pore space (WFPS). The application of 110 kg NH₄⁺-N corresponded to an average of 34 mg NH₄⁺-N kg^-1 dry wt. soil. This was close to the values observed on Day 1 except for a higher value in the treatment with non-placed cattle slurry amended with Vizura (Figure 2, top). With application of non-placed slurry, soil NH₄⁺-N concentrations showed a declining trend during May irrespective of the use of Vizura and then stabilized, but at a higher level in the treatment with Vizura. A different pattern was seen after placement of the slurry (Figure 2, bottom). Here, both treatments showed an increase to around 50 mg NH₄⁺-N kg^-1 in early (unamended slurry) or late May (Vizura-amended slurry). Then followed a period with rapid disappearance in both treatments, but with a 10-14 d delay in the presence of Vizura. The statistical analyses showed significant effects of date and treatment with Vizura on NH₄⁺-N content, but no overall difference between application methods was observed (Table 4).

Following the application of slurry, irrespective of treatment and application method, the NO₃^--N concentration increased gradually during the monitoring period, but with different temporal dynamics. With untreated cattle slurry, the increase was faster during May with placed compared to non-placed slurry, and the final level reached during June was higher, 70-80 mg NO₃^--N kg^-1, with placement of the slurry, compared to 50 mg NO₃^--N kg^-1 with injection (Figure 3). When treated with Vizura, the development of NO₃^--N concentrations were more similar between injected and placed slurry until late May, where the NO₃^--N level plateaued at around 40 mg NO₃^--N kg^-1 with injection but continued to increase to reach 70 mg NO₃^--N kg^-1 with placement of the slurry. There was no overall difference between application methods, but a highly significant effect of treating the slurry with Vizura, and significantly different temporal dynamics with and without Vizura (Table 4).

3.3. Mineral N distribution after placement of slurry

The distribution of NH₄⁺-N and NO₃^--N in the soil profile with placed slurry was determined after 2, 10 and 18 days (Figure 4); the results for untreated and Vizura-treated slurry are summarized by depth interval in Figure 5, and by distance from the row in Figure 6. There was some variability in NH₄⁺-N concentrations that probably reflected heterogeneity in how the soil structure broke and voids filled with slurry during placement. Ammonium was concentrated at 10-20 cm depth, as expected, and remained at the same level during the 18-d period. Some accumulation of NO₃^--N was seen at all depths, but a reduction of NO₃^--N accumulation with Vizura was only recorded in the 5-20 cm layers and not at 0-5 cm depth receiving little or no slurry with placement (Figure 5). The width of the injection tine used for placement of slurry was 26 cm, and in accordance with this the NH₄⁺-N concentrations at 20 cm distance from the row were low and changed little between day 2 and day 18 (Figure 6). By day 18, there was a trend of NO₃^--N accumulation where untreated cattle slurry had been placed, whereas in the treatment with Vizura the accumulation was similar at all distances.

3.4. N₂O emissions

The temporal dynamics of N₂O emissions are shown in Figure 7, with separate plots for within-row (Row) and inter-row (IR) positions, and results of an analysis of variance are shown in Table 5. Trends were similar but with a tendency for higher emissions from with-in row sampling positions. During May there was a gradual increase in N₂O emissions after injection both with and without placement of slurry without Vizura, but from end of May/early June this trend was reversed, and N₂O emissions declined during June and were close to zero at the last sampling.

The N₂O emissions after application of Vizura-treated slurry remained low during most of May, and then followed by a period increasing emissions from both within-row and inter-row positions. This increase was greater with placed compared to non-placed slurry (Figure 7). The N₂O emissions from Vizura-treated slurry also declined and were close to zero at the last sampling. During June, N₂O emissions were significantly higher from treatments with Vizura-amended slurry compared to untreated slurry, as indicated by the analysis of variance and pair-wise comparisons (Table 5). For inter-row positions there was a significant interaction (p < 0.001) between slurry application method and treatment with Vizura (Table 5).

The cumulative N₂O emissions during the monitoring period are shown in Figure 8, and the associated analysis of variance in Table 6. This analysis was based on average emissions from inter-row and within-row positions. Nitrous oxide emissions differed significantly between application methods and was higher from non-placed compared to placed cattle slurry. However, there was a strong interaction (p < 0.001) with slurry treatment, for untreated slurry the N₂O emission was lower from placed slurry. But the opposite pattern was true for slurry treated with Vizura where placed slurry showed higher cumulative N₂O emission during the monitoring period (Figure 8). All treatments except the Control received 221 kg N ha^-1 in cattle slurry plus mineral N fertilizers, and cumulative N₂O emissions constituted 2.8 and 0.3 % of slurry N in non-placed slurry without and with Vizura®, respectively. The N₂O emissions constituted 0.3 and 0.4 % of slurry N without and with the inhibitor in placed slurry.

4. Discussion

Application technique can influence the potential for gaseous losses from liquid manure in the form of ammonia (NH₃) and N₂O emissions, which are, respectively, lower, and higher with incorporation or injection compared to surface application (Hou et al., 2015). The enhanced N₂O emissions from incorporation as opposed to surface application of slurry are likely due to the decomposition of labile organic C in compounds such as volatile fatty acids (Guenzi and Beard, 1981). If the oxygen (O₂) demand by the slurry exceeds the supply, denitrification is enhanced if NO3- is present (Van Groenigen et al., 2014; Rochette et al., 2000). Elevated concentrations of water-soluble C were measured in the slurry injection zone for at least 40 d after application under field conditions (Comfort et al., 1988), and throughout a 20-day incubation experiment with simulated injection (Petersen et al., 1991). The labile C in this environment is probably protected by a higher water content in manure-saturated soil (Petersen et al., 2003), which impedes diffusive transport of O₂ and helps maintain anaerobic conditions. Hence, liquid manure incorporation leads to the development of organic hotspots with a potential for N₂O emissions in the weeks following incorporation (Wagner-Riddle et al., 2020; Velthof and Mosquera, 2011).

Injection (or acidification) of manure is mandatory when applied to bare soil according to local legislation in Denmark (Ministry of Food, Agriculture and Fisheries, 2022). In this study, slurry injection followed by ploughing (non-placement) resulted in higher cumulative N₂O emissions than placement of slurry in a band under crop rows (Figure 8 and Table 6), mainly because of higher emissions from untreated slurry during May. With 25 cm spacing between injection tines and subsequent ploughing, the contact between slurry and soil was probably greater for non-placed slurry compared to the placed slurry applied at 75 cm spacing and with no further disturbance. A larger surface area would promote the exchange of O₂, NH₄⁺ and NO₃^- between manure and soil, and this in turn will stimulate the decomposition of labile C (Sørensen and Jensen, 1995) and support the growth of microbial populations, including nitrifiers and denitrifiers, around manure-soil interfaces (Petersen et al., 1991;1992). The faster onset of NO₃^--N accumulation with untreated non-placed slurry (Figure 3) could thus be due to a higher NH₄⁺ availability for nitrifiers, and more coupled nitrification-denitrification during May enhancing N₂O emissions compared to placed slurry (Figure 7). This interpretation is consistent with the lower initial N₂O emission and effect of the nitrification inhibitor with placed slurry (Figure 7).

Variation in soil temperature or moisture can shift the balance between O₂ supply and demand and result in N₂O emission spikes or multi-day peaks on top of the emission driven by the manure applied (Molodovskaya et al., 2012). In accordance with this, an increase in N₂O emissions was observed with untreated slurry after 20^th May that followed a temperature increase of nearly 10 ◦C and a 12 mm rain event during the previous week (Figure 1). Similarly, there was an increase in N₂O emissions from placed slurry after this change in temperature and rainfall.

Nitrification inhibitors are chemical compounds that reduce primarily bacterial oxidation of ammonia (NH₃) to nitrite (NO₂^-) in fertilized soil (Lei et al., 2022). The addition of nitrification inhibitors has been frequently reported to reduce both N₂O and NO emissions from agricultural soils, although their efficiency depends on soil conditions (Ruser and Schulz, 2015; Guardia et al., 2023). Nitrification inhibitors can reduce N₂O emissions from slurry by desynchronizing C and NO₃^- availability for denitrifiers after field application, but they may be less effective in compacted and/or wet soil with low nitrification activity and a higher potential for complete reduction of NO₃^- to N₂ (Recio et al., 2018; Peixoto and Petersen, 2023). In the present study, treating cattle slurry with DMPP reduced N₂O emissions for non-placed slurry, as hypothesized, and the reduction of 70 % was in line with or higher than previous studies (Misselbrook et al., 2014; Dittert et al., 2001; Menendez et al., 2006). Ammonia oxidation was clearly delayed, but there were higher N₂O emissions from placed slurry treated with DMPP in the last part of the monitoring period (Figure 7), and overall, a non-significant tendency for higher cumulative emissions from this treatment (Figure 8). Therefore, the effect of DMPP on N₂O emissions did not extend into June. The air temperature had increased prior to this period and reached 18 ◦C in mid-June. The rate of nitrification increases with temperature to a maximum at 30 °C in most soils (Subbarao et al., 2006), and whereas DMPP is effective at 5 °C where the inhibitory effect on ammonia oxidation may last for several months (Zerulla et al., 2001). Therefore, inhibitor effectiveness probably declined with increasing temperature. A recent study with pasture soil reported a half-life at 15 ◦C of 12–17 days for DMPP (Chibuike et al., 2022). The increasing rates of NO₃^- accumulation observed may thus have been due to DMPP degradation.

During June the emission of N₂O from placed slurry treated with DMPP was significantly higher than from untreated slurry (Figure 7). Denitrification has been found to be the main source of N₂O emissions from soil amended with cattle slurry (Meng et al., 2022), but the distribution and average concentrations of NO₃^- were similar in placed slurry with and without Vizura®. We propose that the higher N₂O emissions were due to nitrification activity taking place in close association with the slurry treated with Vizura®. By the time the inhibition by DMPP was relieved in June, some O₂ reaching interior parts of the placed slurry allowed nitrification to proceed under O₂ limited conditions, which are known to enhance N₂O emission via ammonia oxidation (Bollmann and Conrad, 1998) or, possibly, nitrifier denitrification (Wrage et al., 2001). It is, however, also possible that nitrifier activity throughout the slurry layer enhanced denitrification activity in nearby anoxic microsites via coupled nitrification-denitrification. A higher NO₃^- availability tends to increase the N₂O:N₂ product ratio of denitrification (Wu et al., 2019), and furthermore a stimulation of N₂O reductase activity (Friedl et al., 2020) could have ceased with disappearance of DMPP; both mechanisms would tend to increase N₂O emissions.

Nitrous oxide emissions approached the background level around the time of the last sampling, but a risk for environmental losses would continue to exist until N uptake was complete (Figure 3). A possible reason for the decline in N₂O emissions is that by this time the pool of reactive C sustaining denitrification had become depleted, and the manure-soil mixture therefore dominated by aerobic decomposition (Petersen et al., 1996).

Placement in a broad band reduced cumulative N₂O emissions compared to non-placed injection. However, the results showed an interaction with respect to the effect of Vizura® on cumulative N₂O emissions, with a reduction when slurry was injected without placement and the opposite trend with placed slurry (Figure 8). The discussion above pointed to a loss of nitrification inhibitor efficiency over time, and to nitrifier activity under oxygen-limited conditions in the manure layer of placed slurry. Unfortunately, the last measurement of soil mineral N distribution took place in mid-May, and the spatial resolution was also insufficient to evaluate this suggestion.

The placement of slurry was expected to improve nutrient availability for the crop. In a separate part of the field study which investigated N and P use efficiency, Pedersen et al. (2022) found that placement of cattle slurry increased the dry matter yield and N uptake of maize at harvest compared to injected slurry, but only in the presence of the nitrification inhibitor. It usually takes several weeks after seeding before the N uptake by maize significantly affects soil mineral N content, and in accordance with this the NO₃^--N concentrations remained constant during the last two weeks of the monitoring period where NH₄⁺ pools had become depleted (Figure 3). The final plateau was higher with placed compared to non-placed slurry irrespective of treatment with the nitrification inhibitor, indicating that environmental N losses had been reduced by placement. However, soil NO₃^- in mid-June was very similar in slurry with and without DMPP and did not suggest a difference in N availability for the crop at this time. Presumably NO₃^- was lost through leaching or gaseous emissions after the monitoring ended, but before crop N uptake was complete, and the potential for loss was higher with non-placed compared to placed slurry, and with untreated compared to Vizura treated slurry.

5. Conclusions

This short-term study investigated effects of cattle slurry distribution in the soil, and effects of a nitrification inhibitor, on soil mineral N dynamics and N₂O emissions. Placement of slurry in a broad band under maize rows reduced N₂O emissions from untreated slurry, possibly because this distribution had less coupled nitrification-denitrification. A nitrification inhibitor, DMPP, reduced N₂O emissions for several weeks with traditional, non-placed slurry application. However, unexpectedly, no effect of DMPP was observed on cumulative N₂O emission after placement of the slurry, where N₂O production from slurry with the inhibitor increased after several weeks. Understanding the interactions between slurry distribution and nitrification inhibitor efficacy will be crucial for recommendations to mitigate N₂O emissions.