Version 1
: Received: 8 September 2023 / Approved: 11 September 2023 / Online: 12 September 2023 (04:43:44 CEST)
How to cite:
Cabral, J.E.; Qiu, Y.; Heck, A.J.; McNulty, R. Differential Large and Small Terminase Subunit Assembly Mediate Bacteriophage P22 Genome Packaging. Preprints2023, 2023090727. https://doi.org/10.20944/preprints202309.0727.v1
Cabral, J.E.; Qiu, Y.; Heck, A.J.; McNulty, R. Differential Large and Small Terminase Subunit Assembly Mediate Bacteriophage P22 Genome Packaging. Preprints 2023, 2023090727. https://doi.org/10.20944/preprints202309.0727.v1
Cabral, J.E.; Qiu, Y.; Heck, A.J.; McNulty, R. Differential Large and Small Terminase Subunit Assembly Mediate Bacteriophage P22 Genome Packaging. Preprints2023, 2023090727. https://doi.org/10.20944/preprints202309.0727.v1
APA Style
Cabral, J.E., Qiu, Y., Heck, A.J., & McNulty, R. (2023). Differential Large and Small Terminase Subunit Assembly Mediate Bacteriophage P22 Genome Packaging. Preprints. https://doi.org/10.20944/preprints202309.0727.v1
Chicago/Turabian Style
Cabral, J.E., Abert J.R. Heck and Reginald McNulty. 2023 "Differential Large and Small Terminase Subunit Assembly Mediate Bacteriophage P22 Genome Packaging" Preprints. https://doi.org/10.20944/preprints202309.0727.v1
Abstract
Concatemeric viral DNA is packaged into Bacteriophage P22 procapsids via a headful packaging mechanism mediated by a molecular machine consisting of small (gp3) and large (gp2) terminase subunits. Although a negative stain reconstruction exists for the terminase holoenzyme, it is not clear how this complex binds dodecameric portal protein located at a 5-fold mismatch vertex. Herein, we describe new assemblies for the holoenzyme. Both native mass spectrometry and transmission electron microscopy reveal that the P22 terminase complex adopts three main assemblies, which include a nonameric S-terminase bound to two L-terminase 1(gp3)9:2(gp2), two nonameric S-terminase bound to five L-terminase 2(gp3)9:5(gp2), and three nonameric S-terminase bound to seven L-terminase 3(gp3)9:7(gp2). Native agarose gel electrophoresis shows that the terminase complex interacts with procapsid with mild crosslinking. These results herein illustrate the P22 terminase complex can adopt a variety of confirmations and assembly states.
Keywords
Salmonella virus; viral genome-packaging motor; small terminase; large terminase; Bacteriophage P22; electron microscopy
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
