Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens

Ahmed R. Elbestawy
* ORCID logo ,
Hany F. Ellakany
ORCID logo ,
Mahmoud E. Sedeik
ORCID logo ,
Ahmed R. Gado
ORCID logo ,
Mervat A. Abdel-Latif
ORCID logo ,
Ahmed E. Noreldin
ORCID logo ,
Ahmed Orabi
,
Ismail A. Radwan
,
Wafaa Abd A. Abd El-Ghany
ORCID logo
Version 1 : Received: 18 August 2023 / Approved: 12 September 2023 / Online: 12 September 2023 (03:43:10 CEST)

A peer-reviewed article of this Preprint also exists.

Elbestawy, A.; Ellakany, H.; Sedeik, M.; Gado, A.; Abdel-Latif, M.; Noreldin, A.; Orabi, A.; Radwan, I.; El-Ghany, W.A. Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens. Vaccines 2023, 11, 1638. Elbestawy, A.; Ellakany, H.; Sedeik, M.; Gado, A.; Abdel-Latif, M.; Noreldin, A.; Orabi, A.; Radwan, I.; El-Ghany, W.A. Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens. Vaccines 2023, 11, 1638.

Abstract

A comparative efficacy of apathogenic genotype I (V4) and lentogenic genotype II (LaSota) strains of live Newcastle disease virus (NDV) vaccines was done following vaccination with pathogen-associated molecular pattern (PAMP) H9N2 avian influenza vaccine and challenge with velogenic NDV genotype VII.1.1 (vNDV-VII.1.1). Eight groups (Gs) of day-old chicks were used (n=25). Groups 1-4 received a single dose of PAMP-H9N2 subcutaneously, while Gs (1, 5) and (2, 6) received eye drops of V4 and LaSota respectively as twice doses. All Gs except 4 and 8 were intramuscularly challenged with vNDV-VII.1.1 at 28th days of age. No signs were detected in Gs 1, 5, 4, and 8. The mortality rates were 0% in Gs 1, 4, 5, and 8; 40% in G2; 46.66% in G6; and 100% in Gs 3 and 7. Lesions were recorded as minimal in Gs 1 and 5, but mild to moderate in Gs 2 and 6. The lowest significant viral shedding was detected in Gs 1, 2, and 5. In conclusion, two successive vaccinations of broilers with a live V4 NDV vaccine provided a higher protection against vNDV-VII.1.1 challenge than LaSota. Besides, PAMP-H9N2 with live NDV vaccines induced more protection than live vaccine alone.

Keywords

vNDV-VII.1.1 challenge; broiler chickens; live ND vaccines; V4; LaSota; PAMP-H9N2

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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