Physiological Functions of Carbon Dots and Their Applications in Agriculture Production

1. Introduction

The world population is constantly growing, and in order to meet the global demand for food and industrial raw materials, the input and consumption of agricultural resources continue to increase. It is estimated that the global annual crop yield is over 3 billion tons, requiring 190 million tons of fertilizers, 4 million tons of pesticides, and 2.7 trillion cubic meters of freshwater resources (accounting for 70% of global freshwater resources). In addition, there is a significant use of pesticides and herbicides, which seriously threatens the global ecosystem and is not conducive to the sustainable development of agriculture [1]. Therefore, there is an urgent need for agriculture to develop new green and environmentally friendly technologies to address the challenges of food growth and sustainable agricultural security development. Nanomaterials are considered to play a crucial role in addressing these challenges faced by future agriculture. In the past few decades, a large number of nanomaterials have been developed and applied in agriculture, playing a vital role in promoting crop growth and development, increasing yield, improving fertilizer and pesticide use efficiency, protecting the ecological environment, and alleviating environmental pressure [2-4].

Carbon dots (CDs) are a type of carbon based nanomaterials with fluorescence properties, consisting of carbon nanoparticles with quasi spherical structures with a size of less than 10 nm [5]. CDs, as the generic term for a variety of nanosized fluores-cent carbon materials, concretely include graphene quantum dots, carbon quantum dots, carbon nanodots, and carbonized polymer dots, which are classified according to the specific carbon core structure, surface groups, and properties, as is shown in Figure 1 [6,7]. CDs generally have O/N-containing functional groups, such as amino (–NH₂), carboxyl (–COOH), and hydroxyl (–OH) on their surface which make these materials water soluble, probably due to the formation of H-bonding [8]. Because CDs are abundant with surface groups, functional ligands can bind to ions, organic molecules, polymers, DNA, and proteins, with the aim to change the properties of the CDs, by tailoring their surface properties and interactions, functionalized CDs can be optimized to meet specific requirements in different fields. Compared with traditional nanomaterials such as cadmium/lead, rare earths, and metal oxides, CDs have advantages in better photostability, higher quantum yield, lower toxicity, abundant low-cost sources, and excellent biocompatibility, which empowers them to have promising applications in biomedicines, optronics, sensors, and catalysis [9]. Recently, many researchers have explored the potential applications of CDs in different aspects, including agriculture [10-16].

Different types of natural raw materials can be employed for the fabrication of CDs, many synthetic methods have been mentioned for the fabrication of CDs [8]. Mass production of CDs can be achieved through technologies such as electrophoresis, laser etching, and electrochemical oxidation [17,18]. Previous studies have explored the effects of carbon based nanomaterials (such as fullerene, carbon nanotubes, and graphene particles) and metal or metal oxide nanoparticles (such as Au, Ag, Fe₃O₄, TiO₂, and ZnO) on the growth of crops [19,20], demonstrating the broad application prospects of nanotechnology in agriculture. CDs, as a new star in carbon based nanomaterials, the research on their physiological function and their impact on crop growth and development are still in its early stages. Since Qu et al. [21] revealed the biocompatibility of CDs with bean sprouts, the potential impact of CDs on the growth of various crops has attracted widespread attention from agricultural researchers. The application effects and mechanisms of CDs in plants, especially crops, need to be further studied, and it is expected to develop new green, high-yield and high-efficiency cultivation technologies that promote crop growth and increase yield, which is of great significance for ensuring global food security.

2. Applications of Carbon Dots in Crop Production

Increasing agricultural productivity is critical to feed the evergrowing human population. In recent years, the promoting effect of CDs on plant growth has been observed in monocotyledonous plants (wheat, rice, and maize), dicotyledonous plants and other plants (mung beans, tomatoes, lettuce, tobacco, soybeans, eggplants, chili peppers, watermelons, radishes, celery, coriander, and cabbage) [5]. CDs are widely used in agriculture as seed priming agents, photosynthetic enhancers, plant stress ameliorators, and sensors [5]. The positive impact of CDs on different plants indicates their great application potential in the agricultural production, which plays an important role in promoting crop growth and improving the sustainability of agricultural production. In Table 1, we summarized the growth enhancement effect of CDs in main cereal crops, rice, wheat and maize, which accounting for 99% of global food production. CDs exhibit dose-dependent and mode of application dependent effects on plants. CDs are introduced into the plant can use various modes of application, such as seed pretreatment, foliar spray, hydroponic solution treatment, and so on. For the full understanding of the effect of CDs on crop development and the underlying mechanism, the evaluation of various growth parameters is involved over a full growth cycle of the plant.

3. The Physiological Role of Carbon Dots and Their Impact on Crop Growth and Development

The primary physiological functions of CDs are to promote seed germination and root growth, improve plant nutrient absorption, promote plant growth, increase biomass accumulation, enhance photosynthesis, increase plant carbohydrate content, plant abiotic stress and disease resistance, these are crucial processes for the growth of plants and increase crop yield (Figure 2).

3.1. The Pathways of Carbon Dots Uptake by Crops and Their Accumulation and Transport Characteristics within Plants

The main pathways for CDs to enter plants include root absorption from soil/water and leaf absorption. Studies have shown that CDs can penetrate plant cells and then be transported along with water and minerals from roots to stems and leaves，hey are absorbed through cell walls and plasmodesmata through extracellular pathways in intercellular spaces and extracellular spaces, and then pass through the cortex and enter the xylem through the plastid pathway [29]. CDs have stable and unique fluorescence signals, providing a good pathway for their tracking in plants. The process of CDs being absorbed by plants can be demonstrated through methods such as fluorescence imaging, transmission electron microscopy, or Raman spectroscopy measurement [20,25,30-32]. Using CDs aqueous solution to cultivate mung beans, concentration dependent red orange fluorescence enhancement can be clearly observed in mung bean seedlings under 365 nm ultraviolet light. The roots, stems, and leaves of young seedlings were observed using confocal laser scanning microscopy, and it was found that CDs mainly exist in the vascular system. Transmission electron microscopy was used to observe the transverse sections of roots, stems, and leaves, and large aggregation clusters of CDs were observed in the intercellular spaces. Therefore, CDs are absorbed by the root and enter the root vascular bundle, which is then transported to the stem and leaf vascular bundles, and then enter the intercellular space for aggregation [33]. In addition, studies on crops such as rice and corn have shown that carbon dots can be absorbed and used by plants through foliar spraying, thereby increasing grain weight and yield [20,26].

3.2. Carbon Dots Enhance Crop Photosynthesis

The growth and yield of crops depend on effective photosynthesis, which is the fundamental material source for plant growth and biomass accumulation, contributing over 90% of crop biomass. One important physiological function of CDs is to enhance plant photosynthesis. Photosynthesis includes two energy conversion processes from light energy to electric energy and from electric energy to chemical energy, involving light absorption, electron transfer, photophosphorylation, carbon assimilation and other important reaction steps. CDs typically exhibit strong absorption in the ultraviolet region (200-400 nm), but their light absorption can be extended to the visible light range due to the type and content of surface groups, as well as changes in oxygen/nitrogen content in carbon nuclei. In the 500-800 nm range, CDs have long wave absorption, converting UV light that cannot be used by plants into visible light [13,34,35]. CDs are both excellent electron donor and electron acceptor [26,36,37], amine functionalized CDs are strongly conjugated on the surface of chloroplasts and assist in absorbing photons to transfer electrons to the chloroplasts, accelerating the all electron transfer chain pathway in photosynthetic reactions, thereby enhancing photosynthesis [38]. 5 mg L^-1 nitrogen doped CDs significantly increased the net photosynthetic rate of maize (21.51%). Further studies have shown that there is an increase in light conversion, electron supply, chlorophyll, ATP synthase (adenosine triphosphate synthase) activity, and NADPH (nicotinamide adenine dinucleotide phosphate) synthesis during photosynthesis, especially a significant increase of 122.80% in electron transfer chain rate [26,28]. In addition, CDs significantly increased the expression of chlorophyll synthase and chlorophyll enzyme genes in rice, which helps to improve chlorophyll synthesis and CO₂ assimilation [22,24]. CO₂ assimilation is a physiological process responsible for the conversion of electrical energy to chemical energy in photosynthesis, and rubisco enzyme is a key enzyme in this process [39]. Rubisco enzyme activity directly affects photosynthetic rate and carbohydrate accumulation. Wang et al. [31] found that the rubisco enzyme activity of mung bean seedlings treated with CDs was 30.9% higher than that of the control. Similar increased effects of CDs on rubisco enzyme activity were found in plants such as rice and arabidopsis [20,40]. In terms of enhancing photosynthesis, CDs have more advantages in monocotyledonous plants compared to dicotyledonous plants. The structural differences in the vascular system and root structure of monocotyledonous and dicotyledonous plants are the reasons for its high photosynthesis [5]. CDs also have different effects on the photosynthesis of C3 plants (rice) and C4 plants (corn), and their effect on the CO₂ assimilation of rice is higher than that of corn, which is attributed to that corn is a C4 plant, it has an internal way to reduce photorespiration and improve the CO₂ fixation rate; CDs also significantly improved the stomatal conductance of rice. The greater the stomatal conductance, the higher the CO₂ absorption in the stomata, therefore, CDs can enhance the gas exchange capacity of plant leaves [23,41]. In addition, CDs can be degraded in plants to form plant hormone analogues and release CO₂, hormone analogues promote plant growth, and CO₂ released is further assimilated through the calvin cycle, thus increasing carbohydrate accumulation [20,40,42]. The above research indicates that CDs have great potential to improve crop growth and photosynthesis in agricultural production. In conclusion, CDs provide artificial photosynthesis support for crops, which increases the photosynthesis rate, and consequently increase grain yield, the morphological charcteristics of plant, such as plant height, biomass, leaf area, and physiologial charcteristics, such as stomata conductance, rubisco activity, ATP and NADP formation, PSI and PSII rate, electron transfer chain are all improved by CDs [43], which rises the carbohydrates levels, and finally increased grain yield.

3.3. Improving Crop Quality with Carbon Dots

Research has shown that CDs can be applied as nanofertilizers, improving crop photosynthesis while also increasing crop yield. Spraying 560 mg L^-1 of CDs aqueous solution on the leaves can increase the yield of dicotyledonous plants such as soybeans, tomatoes, and eggplants by 20% [40]. Wang et al. [31] found that 0.02 mg mL^-1 CDs increased the root length, stem length, root activity, and fresh weight of mung beans, resulting in a 17.5% increase in bean sprout yield compared to the control.

The main source of crop yield is photosynthesis, and the level of yield is determined by the accumulation and distribution of photosynthetic products. Continuous spraying of CDs (50 mg L^-1) during the vegetative growth stage of maize can increase carbohydrate accumulation during the reproductive growth stage, resulting in an increase in 1000-grain weight and a final yield increase of 24.50%. Further research has found that the expression of sucrose transporter (SUT) genes in leaves increased by 1.61 times, and the upregulation of SUT expression enhances the transportation capacity of sucrose in the phloem. Therefore, more carbohydrates are transported from leaves to grains, thereby promoting grain filling and increasing yield [26]. Under drought conditions, spraying nitrogen doped CDs on maize leaves promotes the synthesis of carbohydrates by enhancing photosynthesis, resulting in a 30% reduction in maize yield loss; at the same time, starch, soluble sugar, protein, linoleic acid, and α-linolenic acid content of grains significantly increased by 7.0%, 9.8%, 49.7%, 10.5%, and 12.3%, respectively, thus improving grain quality [28]. The appropriate concentration of nitrogen doped CDs can significantly promote the accumulation of lettuce biomass, significantly improving the soluble sugar and other nutritional quality indicators of lettuce [44].

Fertilizer, as the main source of crop nutrients in modern agricultural production, directly participates in or regulates crop nutrient metabolism and cycling, and is closely related to crop yield and quality. Adding CDs as fertilizer enhancers to different types of fertilizers can accelerate chemical reactions, accelerate nutrient decomposition, improve fertilizer release characteristics, increase fertilizer use efficiency, and promote crop growth and development. Adding nano carbon to slow-release fertilizers can promote the formation of rice tillers, increase chlorophyll content during booting, promote dry matter accumulation, and increase the number of effective panicles and grains per panicle, ultimately leading to increased of grain yield and nitrogen fertilizer use efficiency in rice [45]. Therefore, CDs have great potential in improving crop yield and quality.

3.4. Carbon Dots Promote Seed Germination, Increase Water and Nutrient Absorption

Seed germination is the first and crucial step in plant growth, and good seed germination can help plants develop better. It has been found on both rice and wheat that seeds treated with CDs aqueous solution can promote seed germination [20,21]. Water and nutrient absorption and assimilation are important factors affecting seed germination, crop growth and development, and yield formation. One of the reasons that CDs promote seed germination and enhance seed vitality is that they can penetrate the hard seed coat, promote water infiltration, and facilitate seed water absorption and germination [5]. Seed germination, root development, seed moisture content, and seedling length are related to the surface hydrophilic groups (-OH and -COOH) of CDs. The hydrophilic groups on the surface of CDs provide rich binding sites for water molecules, and it can enter the plant with the absorption of CDs, adequate water absorption promotes seed germination and accelerates seedling growth [20,40]. In addition to serving as adsorption sites for water, CDs upregulate the expression of seed aquaporins genes, activate aquaporins, and reduce rhizosphere pH, which promotes water and nutrient absorption, improves the rhizosphere microbial environment, and is conducive to seed germination and growth [46,47].

The absorption of water by crops is accompanied by the absorption of nutrients. Hydroxyl and carboxyl groups also endow CDs with the ability to adsorb various nutrient ions (K⁺, Ca²⁺, Mg²⁺, Cu²⁺, Zn²⁺, Mn²⁺, and Fe³⁺), which are important nutrient elements for crop growth. They interact with hydrophilic groups on the surface of CDs through hydrogen bonding and electrostatic interactions to adsorb on the surface of CDs, when CDs enter the plant, they also increase the concentration of nutrient ions in the plant, this is also the reason for the sustained and slow release of nutrients in the xylem [48]. The nutrient ion content in arabidopsis treated with CDs is higher than that in the control, indicating that nutrient ions can enter the plant with CDs [40]. Studies on coriander showed that spraying 40 mg L^-1 of CDs increased the element content of K, Ca, Mg, P, Mn, and Fe by 64.3%, 21.0%, 26.2%, 12.8%, 56.0%, and 125%, respectively [49]. When 0.02 mg mL^-1 was used to treat lettuce, the N, P, and K contents in the plants increased by 4.4%, 10.8%, and 16.5%, respectively [50]. On the other hand, after treating with CDs, the expression of genes related to aquaporins (membrane proteins used for transporting water, nutrients, and gases) is upregulated, thereby increasing water and mineral ion absorption and promoting crop growth [51,52]. Carbon element is widely present in soil, and CDs have better environmental friendliness compared to general nanomaterials. After entering the soil, the solubility of CDs increases the EC value of soil. The increase in EC value is the direct reason for the formation of a large amount of bicarbonate ions, promoting the absorption and utilization of water and nutrients such as nitrogen, phosphorus, and potassium by crop roots [53]. In addition, CDs can improve the activity of nitrogenase and the nitrogen fixation efficiency of nitrogen-fixing bacteria by affecting the secondary structure of nitrogenase and improving electron transfer in the biocatalytic process. This provides an economic and environmentally friendly way to improve the biological nitrogen fixation ability of nitrogen-fixing bacteria and provide a nitrogen source for crop growth when nitrogen is insufficient [1,31].

3.5. Carbon Dots Improve Crop Resistance to Abiotic Stress and Disease Resistance

In actual production, crops are constantly challenged by adverse abiotic environmental conditions. According to the report of the Food and Agriculture Organization of the United Nations (FAO) in 2007, more than 96% of the world's rural land is affected by various abiotic stress, including drought, high temperature, low temperature, nutrient deficiency, and excessive salt or heavy metals in the soil. These abiotic stress have a negative impact on crop productivity, leading to serious yield loss [54,55]. For example, drought stress on rice and wheat after flowering can lead to premature plant senescence, reduced material production, shortened filling period, and reduced grain weight [56-58]. CDs can promote plant growth, and a large number of studies have also proved that they can improve plant resistance to abiotic stress, thereby improving crop yield.

The increase of reactive oxygen species (ROS) is the main factor affecting crop growth under abiotic stress. The accumulation of ROS in cells usually leads to oxidative damage to proteins, lipids, carbohydrates, and DNA [59,60]. The mechanism of CDs improving crop resistance to abiotic stress is that CDs have the effect of scavenging free radicals, and the surface of CDs fused with carboxyl and amino groups can transform DPPH free radicals into stable DPPH-H through hydrogen transfer mechanism [61,62]; on the other hand, CDs can increase the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and reduce the contents of ROS and malondialdehyde (MDA) [63]. CDs combine the free radical scavenging characteristics and the ability to improve the activity of antioxidant enzymes to protect crops from abiotic stress, providing a theoretical basis for their application in crop stress resistant cultivation. Research has shown that under drought stress, the application of CDs in maize reduces the accumulation of ROS within the plant, weakens the oxidative stress caused by drought, promotes the synthesis of proline and abscisic acid in the leaves, and long-distance transportation to the roots, thereby upregulating the expression of aquaporin (AQP) genes by 2.3 to 7.6 times, increasing the proportion of K⁺/Na⁺ (by 47.7%), and promoting crop water absorption under drought conditions (by 49.0%); in addition, it increases the transport of carbohydrates to the roots, increases the content of root exudates (organic acids, amino acids, etc.), and increases the abundance of rhizosphere microorganisms (proteobacteria, actinomyces, ascomycetes, etc.), promoting the absorption of nitrogen and phosphorus in maize, thereby improving the drought resistance of crops [28]. CDs also have a positive impact on the growth of soybean under drought conditions, applying 5 mg L^-1 carbon dots on the leaves can eliminate the accumulation of reactive oxygen species in soybean leaves under drought stress, enhancing photosynthesis and carbohydrate transport; on the other hand, CDs stimulate the root to secrete amino acids, organic acids, auxin and other organic substances, recruit beneficial microorganisms for the rhizosphere (such as actinomyces, ascomycetes, acidobacteria and mycobacterium), and promote the activation of soil nitrogen; at the same time, the expression of GmNRT, GmAMT, and GmAQP genes in the root was upregulated, promoting nitrogen absorption, utilization, and metabolism, significantly improving the nitrogen content and water absorption capacity of soybeans; in addition, it also increased the content of protein, fatty acids, and amino acids in soybean seeds by 3.4%, 6.9%, and 17.3%, respectively [64,65]. Wang et al. [66] explored the relieving effect of CDs on heat stress in Italian lettuce. The study showed that CDs enhanced antioxidant enzyme activity and osmoregulation (manifested by a decrease in proline content), and reduced damage caused by lipid peroxidation in plant cells (manifested by a decrease in MDA levels), thereby improving heat tolerance. Soil salt stress is also an important factor affecting plant growth. Salt stress leads to crop nutrient deficiency, osmotic stress, and ion toxicity. Li et al. [67] showed that CDs exhibit Ca²⁺ mobilization characteristics and can alleviate salinity stress by enhancing Ca²⁺ signaling and ROS scavenging activity. In addition, Gohari et al. [68] found that putrescine functionalized CDs can increase K content, photosynthetic pigments, proline, phenolic substances, and antioxidant enzyme activity in grape leaves, while reducing Na⁺, MDA, and H₂O₂ content, thereby alleviating salt stress and increasing leaf fresh and dry weight. The above research provides a theoretical basis for CDs as abiotic stress modifiers to improve crop yield and plant protection.

Crop diseases are important limiting factors that affect crop growth and yield formation. Currently, prevention and control measures mainly rely on the widespread use of pesticides/fungicides, and the inefficient use of pesticides seriously threatens biodiversity and ecosystem functions. Previous study has found that CDs have broad-spectrum antibacterial activity against bacteria and fungi, laying the foundation for their application in crop disease control and improving crop disease resistance [69]. CDs can destroy the secondary structure of DNA/RNA in bacterial walls and bacteria and fungi, thus showing broad-spectrum antibacterial/antifungal activity against gram-positive (staphylococcus aureus and bacillus subtilis) and gram-negative (bacillus subtilis and escherichia coli) bacteria [1,20]. Luo et al. [70] found that foliar spraying of 10 mg L^-1 nitrogen doped CDs activated the acquired resistance of jasmonic acid and salicylic acid dependent systems in tomato, resulting in the stagnation of pathogen growth in vivo, effectively reducing the symptoms of tomato green wilt syndrome caused by ralstonia solanacearum by 71.19%. Therefore, CDs can be used as green and efficient antibacterial agents for preventing and controlling of crop disease.

3.6. The Protective Effect of Carbon Dots on Agricultural Ecological Environment

A good ecological environment is the key to food security production. Given the low toxicity, chemical inertness, biodegradability, and low cost of CDs, they are considered as an excellent choice for degrading organic pollutants. Pesticides, industrial chemicals, by-products of industrial processes, and aromatic hydrocarbons entering the environment can induce and accumulate pathogenic bacteria in the food chain, transforming them into organic pollutants. These pollutants have mutagenic, carcinogenic, and endocrine disrupting chemical properties, which harm human health. Existing research has recognized the key role of CDs in the photodegradation of organic pollutants such as residual pesticides and endocrine disruptors, Cruz et al. [71] found that CDs were used in combination with a photosensitizer for photodegradation of organic dyes and toxic gases. Compared with their original photocatalysts, the addition of CDs greatly improved the degradation rate. Carbon dots have the potential to be used as photocatalysts for tetracycline degradation. Tetracycline is a broad-spectrum antibiotic, which can promote the growth of livestock and poultry when added in low doses, and can be used to treat animal diseases when used in high doses. Because its massive use in livestock and poultry and aquaculture causes certain residues in the environment, it is a more prominent environmental risk problem. Recent research shows that, without any additives, nitrogen doped carbon dots can completely degrade tetracycline within 10 minutes, thereby eliminating its adverse effects on the ecological environment [72]. In addition, CDs can also reduce the absorption of heavy metal ions by crops, thereby avoiding the harm of heavy metals to crop growth [73], Because heavy metal ions can be trapped between functional groups on the surface of CDs, reducing the concentration of heavy metal ions in crops [74]. The addition of CDs has a certain activation effect on soil urease, dehydrogenase, and catalase, changing soil pH, increasing soil microbial community abundance and diversity, increasing Cd²⁺ fixation, reducing Cd²⁺ mobility, and thus reducing the harm of Cd²⁺ to the environment [75]. Safe water is the fundamental needs that promote healthy crop production. But the massive and continuously increasing consumption of hazardous chemicals at the domestic and industrial level has led to large amount of wastewater discharge and ecological problems. The green synthesized carbon-dots-based photocatalysts for wastewater treatment have the advantages of inexpensive, non-toxic, require basic ingredients, exhibit quick reactions, involve easy procedures and straightforward post-processing steps [8,76,77] . The understanding of carbon-dots-based photocatalytic degradation of contaminated wastewater will also set the stage for future wastewater treatment research.

3.7. The Role of Carbon Dots as Biosensors in Agriculture

Due to the fluorescence quenching phenomenon, as the concentration of pesticides increases, the fluorescence intensity of CDs significantly decreases. Based on this, Tafreshi et al. [78] developed a fluorescence based method for the detection of three pesticides, including diazinon, glyphosate, and semicarbazide. The sensor has good selectivity even in the presence of other herbicides, and has excellent performance in tomato samples. It can also be used in other agricultural products to achieve ultra sensitive fluorescence detection of pesticides in real samples using green carbon dots. Research has shown that when electrostatic interactions occur between functional groups on the surface of CDs and isothio pesticides, CDs exhibit fluorescence quenching, Ghosh et al. [79] developed a sensor for the detection of isothio pesticides based on the fluorescence quenching phenomenon of CDs. The detection limit is as low as nanomolar concentrations in water, as well as in fruit and rice samples, and it still has high selectivity in the presence of various interfering substances and other pesticides. The fluorescence properties of CDs can be significantly regulated through atomic doping or surface functionalization. Doped CDs can be single element doped or multi element doped, which enhances the fluorescence characteristics of CDs and significantly improves their performance in sensing applications [80,81]. Yahyai et al. [82] synthesized sulfur and nitrogen doped CDs, a paper based chemiluminescence sensor was developed for the determination of oxfamil pesticide in fruit juice and water. It was found that KMnO₄ oxidized S, N-CDs and produced their excited state, which significantly enhanced the luminous intensity of CDs. Therefore, the sensitivity and detection capability of the sensor were significantly improved. In addition, the sensor showed a good recovery rate between 97.5 and 105.5 in actual samples, which indicates the high performance of the sensor. Luo et al. [83] developed B, N doped CDs and fluorescence platforms based on cerium oxide (CeO₂), CeO₂ showed catalytic reaction of p-methyl-p-oxyphosphorus in alkaline medium, converting it into p-nitrophenol and phosphate, reducing the fluorescence activity of B, N-CDs, which can be used to detect the residues of methyl-p-oxyphosphorus pesticides in herbal plants and water. CDs also can be used for wastewater remediation by sensing of heavy-metal ions (e.g., Pb²⁺, Hg²⁺, Cr⁶⁺, Cd²⁺, or Cu²⁺) and various anions in water bodies [7], which offer feasible strategy for the safe crop production and the protection of human health and the environment.

4. Futuristic Outlook

Although considerable developments have been made in research on CDs, further research is needed to broaden the understanding of their potential application in agriculture. Here, future research prospects of CDs in agriculture will be discussed in brief (Figure 3).

Low and middle yield farmland in China accounts for 90% of the total arable land in the country. Improving the crop yield of medium and low yield farmland is an important issue that urgently needs to be solved in food production of China, as well as an important way to implement the strategy of "storing grain in the land" and ensure food security of China [84]. The traditional methods of improving soil by increasing the application of nitrogen, phosphorus, potassium, organic fertilizers, biochar, etc. require fossil energy consumption, high cost and energy consumption, and the soil water and fertilizer retention capacity is generally poor in medium and low yield fields, which is prone to nutrient loss, environmental pollution, and is not conducive to sustainable development of agricultural production, making it difficult to promote on a large scale in production. Given the low-cost, non-toxic, and excellent physiological effects of carbon dots, it is of great significance to regulate them from the perspective of crops themselves. In the future, we will increase research on the impact of carbon dots on the growth, development, and yield formation of different crops under medium and low yield field conditions, and develop new environmentally friendly cultivation and regulation technologies to improve the production capacity of medium and low yield fields and food security in China.

The non-structural carbohydrates stored in the stems and sheaths of cereal crops (such as rice, wheat, and corn) are important sources of grain filling substances, and their transport rate and amount affect the grain filling rate, thereby affecting grain weight and yield [85,86]. Vascular bundle development, hormone levels, carbon metabolism enzyme activity, sucrose transporter and SWEET gene expression and protein levels are important factors affecting photosynthetic transport and grain filling [87-90]. Carbon dots were found to upregulate the expression of sucrose transport protein genes in maize, which may enhance the sucrose transport ability of the phloem, thereby increasing the transport of photosynthetic assimilates to grains, promoting grain filling and yield formation [20,26]. Our recent field experiments have shown that under ruduced nitrogen input conditions, foliar spraying of CDs on rice can simultaneously improve leaf net photosynthetic rate, stem non-structural carbohydrates translocation, seed setting rate, and grain yield (data not published). Therefore, further research on the regulatory role and mechanism of CDs on stem has important guiding significance for green, high yield, efficient, and high-quality cultivation of rice.

Previous studies have confirmed that CDs have antibacterial effects and can enhance plants' ability to resist plant pathogens and pests, but they may also pose a potential threat to beneficial microbial communities. Therefore, it is necessary to evaluate their potential impact on non target organisms to ensure their safe application in agriculture. In addition, the impact of CDs on crops depends on the application dosage and varies from crop to crop. Numerous studies have reported the promotion of CDs on crop growth. However, high doses of CDs can also have adverse effects on plant growth, such as 1000 to 2000 mg L^-1 CDs significantly reducing the fresh weight of roots and aboveground parts of seedlings in maize [30]. CDs cause a decrease in the growth rate of microalgae in suspension, resulting in a decrease in available light flux due to their absorption of light (shading effect) [91]. Therefore, dosage optimization is necessary for different crops before applying CDs, in order to improve their application effectiveness in large-scale production. In addition, existing research lacks sufficient understanding of the bioaccumulation of CDs in animals and plants and their subsequent cumulative effects on entering the food chain. Therefore, conducting systematic and in-depth research in this field will elucidate the ecological toxicity of CDs and contribute to their safe application in agriculture.

Sensors based on CDs have great prospects in agricultural monitoring, and research in this field is still in its early stages. The previously reported CDs based sensors are excellent candidates for detecting various pesticide residues in agricultural products, including insecticides and herbicides. In this field, further research is still needed to accelerate the construction of a highly sensitive sensor platform for rapid sensing of food and other products, and expand to the development of an integrated, convenient and wearable sensor to achieve real-time and on-site detection of toxic chemicals and heavy metals, and real-time and on-site monitoring of plant growth, micronutrients, and abiotic and Biotic stress of plants, contribute to the development of efficient agriculture in the future.

In addition, the easy surface functionalization of CDs indicates that they can serve as promising carriers to carry specific molecules into crops, such as insecticides, nutrients, plant growth regulators, etc., thereby achieving precise regulation of crop growth and development.