Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Galleria mellonella Antimicrobial Peptides and Stress Management Gene Expression in Response to Deleterious Events Caused by Sporothrix brasiliensis

Nathália Faria Reis
,
Myrela Conceição Santos De Jesus
,
Lais Cavalcanti dos Santos Velasco De Souza
ORCID logo ,
Lucas Martins Alcântara
,
Julia Andrade de Castro Rodrigues
,
Simone Cristina Pereira Brito
,
Patrícia Azambuja
,
Cecília Stahl Vieira
,
José Rodrigo Santos Silva
ORCID logo ,
Bruno de Araújo Penna
ORCID logo ,
Ricardo Luiz Dantas Machado
ORCID logo ,
Héctor M. Mora-Montes
ORCID logo ,
Andréa Regina Souza Baptista
* ORCID logo
Version 1 : Received: 31 August 2023 / Approved: 1 September 2023 / Online: 4 September 2023 (07:11:48 CEST)

A peer-reviewed article of this Preprint also exists.

Reis, N.F.; de Jesus, M.C.S.; de Souza, L.C.S.V.; Alcântara, L.M.; Rodrigues, J.A.C.; Brito, S.C.P.; Penna, P.A.; Vieira, C.S.; Silva, J.R.S.; Penna, B.A.; Machado, R.L.D.; Mora-Montes, H.M.; Baptista, A.R.S. Sporothrix brasiliensis Infection Modulates Antimicrobial Peptides and Stress Management Gene Expression in the Invertebrate Biomodel Galleria mellonella. J. Fungi 2023, 9, 1053. Reis, N.F.; de Jesus, M.C.S.; de Souza, L.C.S.V.; Alcântara, L.M.; Rodrigues, J.A.C.; Brito, S.C.P.; Penna, P.A.; Vieira, C.S.; Silva, J.R.S.; Penna, B.A.; Machado, R.L.D.; Mora-Montes, H.M.; Baptista, A.R.S. Sporothrix brasiliensis Infection Modulates Antimicrobial Peptides and Stress Management Gene Expression in the Invertebrate Biomodel Galleria mellonella. J. Fungi 2023, 9, 1053.

Abstract

Sporothrix brasiliensis is the most pathogenic species, responsible for the Brazilian cat-transmitted sporotrichosis hyperendemics. In this scenario, investigation of pathogen-host interaction can provide relevant information for future treatment strategies. To this end, the invertebrate Galleria mellonella has proven to be a suitable alternative to evaluate the virulence of pathogenic fungi since the insect immune system is similar to the mammalian innate immune response. The aim of this work was to investigate phenotypic and molecular aspects of the immune response of G. mellonella throughout the S. brasiliensis infection. Hemocyte density and the evolution of the fungal load were evaluated. In parallel, RT- qPCR expression analysis of genes encoding antimicrobial peptides (Gallerimycin and Galiomycin) and stress management genes (C7 Contig 15362 and C8 Contig 1910) was conducted. Fungal load and hemocyte densities were proportionally increasing simultaneously to the deleterious morphological events and larvae mortality. Gallerimycin, C7 Contig 15362, and C8 Contig 19101 genes were positively regulated (p<0.05) at distinct moments of S. brasiliensis infection, characterizing a time-dependent and alternately modulated profile. Galiomycin gene expression remained unchanged. Our results contribute to the future proposal of potential alternative pathways to treat and, consequently, control S. brasiliensis zoonosis, a major public Health in Latin America.

Keywords

Sporotrichosis; Gene Expression; Invertebrates; Antimicrobial Peptides; Zoonosis; Immune response

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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