Humanized and Defucosylated Antibody against Podoplanin (humLpMab-23-f) Exerted Antitumor Activities in Human Tumor Xenograft Models

Hiroyuki Suzuki; Tomokazu Ohishi; Mika K. Kaneko; Yukinari Kato

doi:10.20944/preprints202308.1756.v2

Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 24 August 2023 / Approved: 24 August 2023 / Online: 24 August 2023 (15:18:44 CEST)
Version 2 : Received: 20 October 2023 / Approved: 20 October 2023 / Online: 20 October 2023 (12:26:35 CEST)

We previously developed a highly sensitive and specific anti-PDPN mAb, LpMab-23 (mouse IgG1, kappa). In this study, we produced a humanized IgG1 version (humLpMab-23) and its defucosylated form (humLpMab-23-f) of an anti‑PDPN mAb to potentiate the ADCC activity. The humLpMab-23 could recognize PDPN‑overexpressed Chinese hamster ovary (CHO)-K1 (CHO/PDPN) and PDPN-positive PC-10 and LN319 cells by flow cytometry. Furthermore, we found that humLpMab-23-f exerted ADCC and complement-dependent cytotoxicity against CHO/PDPN, PC-10 and LN319 cells in vitro and exhibited potent antitumor activities in the xenograft models. These results indicated that humLpMab-23-f could be useful for an antibody treatment regimen for PDPN-positive human cancers.

PDPN; lung cancer; glioblastoma; monoclonal antibody; antitumor activities; mouse xenograft model; antibody-dependent cellular cytotoxicity

Medicine and Pharmacology, Oncology and Oncogenics

Comment 1

Received: 20 October 2023
Commenter: Hiroyuki Suzuki
Commenter's Conflict of Interests: Author

Comment: We changed, as follows.

2.2. Recombinant mAb production

human lambda chain >>> human kappa chain

Figure 2 legend

We revised graphic alphabetic numbers (A~D) to match the description.

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Humanized and Defucosylated Antibody against Podoplanin (humLpMab-23-f) Exerted Antitumor Activities in Human Tumor Xenograft Models

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