Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Anti-fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis. An Ex Vivo Study

Natacha Malu Miranda Da Costa
,
Ludovica Parisi
,
Benedetta Ghezzi
ORCID logo ,
Lisa Elviri
ORCID logo ,
Sergio Luis Scombatti de Souza
,
Arthur Belém Novaes Jr.
ORCID logo ,
Paulo Tambasco De Oliveira
,
Guido Maria Macaluso
ORCID logo ,
Daniela Bazan Palioto
* ORCID logo
Version 1 : Received: 15 August 2023 / Approved: 16 August 2023 / Online: 17 August 2023 (13:14:36 CEST)

A peer-reviewed article of this Preprint also exists.

da Costa, N.M.M.; Parisi, L.; Ghezzi, B.; Elviri, L.; Scombatti de Souza, S.L.; Novaes Júnior, A.B.; de Oliveira, P.T.; Macaluso, G.M.; Palioto, D.B. Anti-Fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis: An Ex Vivo Study. Biomimetics 2023, 8, 582, doi:10.3390/biomimetics8080582. da Costa, N.M.M.; Parisi, L.; Ghezzi, B.; Elviri, L.; Scombatti de Souza, S.L.; Novaes Júnior, A.B.; de Oliveira, P.T.; Macaluso, G.M.; Palioto, D.B. Anti-Fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis: An Ex Vivo Study. Biomimetics 2023, 8, 582, doi:10.3390/biomimetics8080582.

Abstract

Background: Scaffold (SCA) functionalization with aptamers (APT) provides adsorption of specific bioactive molecules on biomaterials surface. The aim of this study was to observe if SCA enriched with anti-fibronectin APT can favor coagulum (PhC) and osteoblasts (OSB) differentiation. Methods: 20 ug of APT was functionalized on SCA by simple adsorption. For PhC formation, SCAs were inserted into rat calvaria defects for 17 hours. Following proper transportation (Buffer solution-PB), OSB (UMR-106 lineage) were seeded over PhC + SCAs with and without APT. Cells and PhC morphology, PhC cell population, protein labelling and gene expression were observed in different time points. Results: The APT induced higher ALP and BSP immunolabeling in OSB. CD90, CD45 and CD44 expression was more detected in APT group than when scaffolds were not functionalized. Additionally, an enriched and dense fibrin network and different cell types were observed, with more OSB and white blood cells in PhC formed on SCA with APT. The gene expression showed higher TGF-β1 detection in SCA with APT. Conclusions: The SCA functionalization with fibronectin aptamers provides a selective expression of proteins related to coagulation pattern and osteo differentiation. Additionally, aptamers increase TGF-β1 gene expression, which is highly associated with improvements in regenerative therapies.

Keywords

Aptamer; Blood Clot; Three dimensional; Scaffold; Hydrogel; Osteogenesis.

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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