Version 1
: Received: 14 August 2023 / Approved: 15 August 2023 / Online: 16 August 2023 (07:52:10 CEST)
Version 2
: Received: 16 August 2023 / Approved: 16 August 2023 / Online: 17 August 2023 (14:32:51 CEST)
Version 3
: Received: 17 August 2023 / Approved: 18 August 2023 / Online: 21 August 2023 (10:34:44 CEST)
How to cite:
MohammadNejad Aynede, A.; Keshani, K.; Mohsenzadeh, S.; Eslami-Farouji, A. Analysis of AP2/ERF Domain of DREB Transcription Factor in Several Wheat Species. Preprints2023, 2023081160. https://doi.org/10.20944/preprints202308.1160.v3
MohammadNejad Aynede, A.; Keshani, K.; Mohsenzadeh, S.; Eslami-Farouji, A. Analysis of AP2/ERF Domain of DREB Transcription Factor in Several Wheat Species. Preprints 2023, 2023081160. https://doi.org/10.20944/preprints202308.1160.v3
MohammadNejad Aynede, A.; Keshani, K.; Mohsenzadeh, S.; Eslami-Farouji, A. Analysis of AP2/ERF Domain of DREB Transcription Factor in Several Wheat Species. Preprints2023, 2023081160. https://doi.org/10.20944/preprints202308.1160.v3
APA Style
MohammadNejad Aynede, A., Keshani, K., Mohsenzadeh, S., & Eslami-Farouji, A. (2023). Analysis of AP2/ERF Domain of DREB Transcription Factor in Several Wheat Species. Preprints. https://doi.org/10.20944/preprints202308.1160.v3
Chicago/Turabian Style
MohammadNejad Aynede, A., Sasan Mohsenzadeh and Atena Eslami-Farouji. 2023 "Analysis of AP2/ERF Domain of DREB Transcription Factor in Several Wheat Species" Preprints. https://doi.org/10.20944/preprints202308.1160.v3
Abstract
The Apetala2/ethylene response factor superfamily refers to a group of transcription factors that share a conserved AP2 DNA binding domain. These factors have been found to have different roles in plant responses to both biotic and abiotic stresses. Samples of hexaploid wheat, tetraploid pasta (or durum wheat), and diploid wheat progenitors were selected. The 29 dehydration-responsive element binding transcription factors in these samples were downloaded from NCBI GenBank for six different countries: Iran, China, Italy, France, Afghanistan, and Azerbaijan. The AP2 domain sequences were identified from the dehydration-responsive element binding transcription factors using PROSITE, ProDom, and SMART software. Next, all sequences were aligned using Multalin and Jalview software. The aligned sequences were then analyzed to identify amino acid locations, types, and frequencies. The multiple alignments showed that approximately 76% of the amino acid residues in the AP2 domains are conserved. According to the amino acid analysis, alanine, serine, and glutamic acid are the most abundant amino acids found in three motifs. The performed phylogenetic analysis illustrates the role of geographical effects on the transcription factor sequences of bread wheat in the Middle East. Significant differences were found between Iranian and Chinese transcription factor sequences. Moreover, genetic variation was observed in the transcription factors of Italian sequences found in pasta and wheat progenitors. Motif structures play a critical role in the domain organization of wheat proteins to enhance the characteristics of assorted metabolic pathways. The structure of the AP2 domain was analyzed by several programs, I-TASSER for instance, to identify the α-helix, β-sheets, and the regions of some significant amino acids in the 3-D model.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter: AmirHossein MohammadNejad Aynede
Commenter's Conflict of Interests: Author
- edited the affiliation section, and Table 1