Version 1
: Received: 12 August 2023 / Approved: 14 August 2023 / Online: 15 August 2023 (09:09:22 CEST)
Version 2
: Received: 19 September 2023 / Approved: 19 September 2023 / Online: 19 September 2023 (16:39:01 CEST)
Eldaghayes, I.; Rothwell, L.; Skinner, M.; Dayhum, A.; Kaiser, P. Efficacy of Fowlpox Virus Vector Vaccine Expressing VP2 and Chicken Interleukin-18 in the Protection against Infectious Bursal Disease Virus. Vaccines 2023, 11, 1716. https://doi.org/10.3390/vaccines11111716
Eldaghayes, I.; Rothwell, L.; Skinner, M.; Dayhum, A.; Kaiser, P. Efficacy of Fowlpox Virus Vector Vaccine Expressing VP2 and Chicken Interleukin-18 in the Protection against Infectious Bursal Disease Virus. Vaccines 2023, 11, 1716. https://doi.org/10.3390/vaccines11111716
Eldaghayes, I.; Rothwell, L.; Skinner, M.; Dayhum, A.; Kaiser, P. Efficacy of Fowlpox Virus Vector Vaccine Expressing VP2 and Chicken Interleukin-18 in the Protection against Infectious Bursal Disease Virus. Vaccines 2023, 11, 1716. https://doi.org/10.3390/vaccines11111716
Eldaghayes, I.; Rothwell, L.; Skinner, M.; Dayhum, A.; Kaiser, P. Efficacy of Fowlpox Virus Vector Vaccine Expressing VP2 and Chicken Interleukin-18 in the Protection against Infectious Bursal Disease Virus. Vaccines 2023, 11, 1716. https://doi.org/10.3390/vaccines11111716
Abstract
In mammals, the role of interleukin-18 (IL-18) in the immune response is to drive inflammatory and, normally therefore, anti-viral responses. IL-18 also shows promise as a vaccine adjuvant in mammals. Chicken IL-18 (chIL-18) has been cloned. The aim of this study was to investigate the potential of chIL-18 to act as a vaccine adjuvant in the context of a live recombinant Fowlpox virus vaccine (fpIBD1) against Infectious bursal disease virus (IBDV). fpIBD1 protects against mortality, but not against damage to the bursa of Fabricius caused by IBDV infection. The Fowlpox virus genome itself contains several candidate immunomodulatory genes, including potential IL-18 binding proteins (IL-18bp). We have knocked out (Δ) the potential IL-18bp genes in fpIBD1 and inserted (::) the cDNA encoding chIL-18 into fpIBD1 in the non-essential ORF030, generating five new viral constructs –fpIBD1::chIL-18, fpIBD1ΔORF073, fpIBD1ΔORF073::chIL-18, fpIBD1ΔORF214 and fpIBD1ΔORF214::chIL-18. The subsequent protection from challenge with virulent IBDV, as measured by viral load and bursal damage, given by these altered fpIBD1 strains, was compared to that given by the original fpIBD1. The results show that chIL-18 can act as a vaccine adjuvant, giving complete protection against challenge, with no detectable virus or damage in the bursa of Fabricius.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter: Ibrahim Eldaghayes
Commenter's Conflict of Interests: Author