Rostami, N.; Ghebleh, A.; Noei, H.; Salimian, Z.; Moeinzadeh, A.; Eslami, S.S.; Gomari, M.M.; Tarighi, P.; Uversky, A.V.N. PCL-PEG Nanoparticle Functionalized with A6 Peptide: A Promising Delivery System for Curcumin Against Cancer Cells. Preprints2023, 2023081057. https://doi.org/10.20944/preprints202308.1057.v1
APA Style
Rostami, N., Ghebleh, A., Noei, H., Salimian, Z., Moeinzadeh, A., Eslami, S.S., Gomari, M.M., Tarighi, P., & Uversky, A.V.N. (2023). PCL-PEG Nanoparticle Functionalized with A6 Peptide: A Promising Delivery System for Curcumin Against Cancer Cells. Preprints. https://doi.org/10.20944/preprints202308.1057.v1
Chicago/Turabian Style
Rostami, N., Parastoo Tarighi and And Vladimir N. Uversky. 2023 "PCL-PEG Nanoparticle Functionalized with A6 Peptide: A Promising Delivery System for Curcumin Against Cancer Cells" Preprints. https://doi.org/10.20944/preprints202308.1057.v1
Abstract
Polymeric based nanoparticles are garnering significant interest for their potential in drug delivery. Specially, nanopolymers that have been functionalized with molecules, such as proteins or peptides, are a versatile vehicle for drug delivery. Curcumin (Cur) is one of the most commonly studied anticancer compounds and is widely acknowledged as having positive effects on human health. However, its insolubility and low bio-distribution greatly hinder the exploitation of its beneficial traits. In this study, our team created a nano delivery system which utilized a nanopolymer called PCL-PEG (poly(ε-caprolactone)-poly (ethylene glycol)). This system was functionalized with A6 peptide to enable targeted delivery of Cur. The system was designed with advantageous nanoparticles (NPs), including a stable zeta potential (–32.7), small size (54.3), uniform surface morphology, and high hydrophilicity (13.72°). In addition, targeted delivery systems exhibited high encapsulation efficacy (93 ± 0.89 %), and drug loading (16.7 ± 0.9 %), and were characterized by a slow and gradual release profile with a release of approximately 83.13 ± 0.12%. The MTT assay results showed that the formulation known as Cur-NPs-A6 caused a significant increase in cell death in MDA-MB-231 cancer cells (IC50 = 23.31 ± 0.85 µM). The designed delivery system did not cause any harm to the noncancerous MCF-10A cells (normal group). Furthermore, the results of both the RT-qPCR and invasion assays demonstrated that the designed system was able to effectively activate the apoptosis pathway and inhibit cell invasion. Our findings suggest that the use of the designed smart delivery system, which is functionalized with A6 peptide that has a high affinity for CD44 receptor - known to be upregulated in numerous malignant conditions, could be a highly efficient approach to treating cancer.
Keywords
Targeted delivery; A6 peptide; PCL-PEG; Nanoparticle; Nanopolymer; Curcumin; Cancer
Subject
Chemistry and Materials Science, Polymers and Plastics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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