Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Validation and cross-species transferability of SSR markers developed from whole genome sequence (WGS) of Mungbean (Vigna radiata L. Wilczek) and their application of population genetics in Vigna species

Pawan Saini
ORCID logo ,
Tejinderjit Singh Bains
,
Asmita Sirari
,
Inderjit Singh Yadav
,
Kamalpreet Singh Mandahal
,
Sayeed Abdul Hamid Patel
,
Javed Akhatar
* ORCID logo ,
Navkiran Ludhar
,
Pooja Saini
,
Sharon Nagpal
,
Aditya Pratap
Version 1 : Received: 7 August 2023 / Approved: 8 August 2023 / Online: 8 August 2023 (11:23:00 CEST)

How to cite: Saini, P.; Bains, T.S.; Sirari, A.; Yadav, I.S.; Mandahal, K.S.; Patel, S.A.H.; Akhatar, J.; Ludhar, N.; Saini, P.; Nagpal, S.; Pratap, A. Validation and cross-species transferability of SSR markers developed from whole genome sequence (WGS) of Mungbean (Vigna radiata L. Wilczek) and their application of population genetics in Vigna species. Preprints 2023, 2023080658. https://doi.org/10.20944/preprints202308.0658.v1 Saini, P.; Bains, T.S.; Sirari, A.; Yadav, I.S.; Mandahal, K.S.; Patel, S.A.H.; Akhatar, J.; Ludhar, N.; Saini, P.; Nagpal, S.; Pratap, A. Validation and cross-species transferability of SSR markers developed from whole genome sequence (WGS) of Mungbean (Vigna radiata L. Wilczek) and their application of population genetics in Vigna species. Preprints 2023, 2023080658. https://doi.org/10.20944/preprints202308.0658.v1

Abstract

The genus Vigna is penta-tropical having more than 200 species with many desirable economically important traits. The aim of study was to validate the in-silico polymorphism of whole genome sequence developed mungbean specific SSR markers and their transferability among the different Vigna species. The present study utilized a set of 200 SSR markers developed from whole genome sequence of mungbean and validated using a diversity panel of 25 accessions which belongs to 13 Vigna species. Out of 200 SSR markers, 130 markers (65%) were polymorphic across the various Vigna species and the number of alleles amplified varied between 7 to24. The SSR markers showed more than 90 percent transferability across the different Vigna species accessions. Based on allelic data, the 25 Vigna accessions grouped into three clusters based upon the unweighted pair group method with arithmetic mean (UPGMA) phylogenetic tree. The principal co-ordinates analysis (PCA) biplot graph and UPGMA based neighbor joining clustering diagram showed similar pattern of Vigna accessions distribution. The population structure assessment has grouped the cultivated and wild species accessions into two sub-population. The estimated marker parameters such as polymorphic information content (0.09 – 0.84), marker index (0.091 – 3.342) and effective multiplex ratio (1.0 – 4.0) suggested their adequacy in several genetic studies such as parental selection, hybrid testing, genetic mapping and marker aided breeding programmes for genetic enhancement of species belonging to the Vigna genus.

Keywords

Mungbean, Vigna, Whole genome sequence, SSR markers, Principal co-ordinate analysis, Genetic diversity

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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