Eszterbauer, E.; Németh, Á. Investigations for a Yarrowia-Based Biorefinery: In Vitro Proof-of-Concept for Manufacturing Sweetener, Cosmetic Ingredient, and Bioemulsifier. Fermentation2023, 9, 793.
Eszterbauer, E.; Németh, Á. Investigations for a Yarrowia-Based Biorefinery: In Vitro Proof-of-Concept for Manufacturing Sweetener, Cosmetic Ingredient, and Bioemulsifier. Fermentation 2023, 9, 793.
Eszterbauer, E.; Németh, Á. Investigations for a Yarrowia-Based Biorefinery: In Vitro Proof-of-Concept for Manufacturing Sweetener, Cosmetic Ingredient, and Bioemulsifier. Fermentation2023, 9, 793.
Eszterbauer, E.; Németh, Á. Investigations for a Yarrowia-Based Biorefinery: In Vitro Proof-of-Concept for Manufacturing Sweetener, Cosmetic Ingredient, and Bioemulsifier. Fermentation 2023, 9, 793.
Abstract
Yarrowia lipolytica is a widely used microorganism in biotechnology since it is capable of producing a wide range of products. A less studied related strain is Y. divulgata, which is also capable of erythritol production in even higher concenctration then most Y. lipolytica wild strains. Thus the aim of this work was to investigate Y. divulgata’s complex utilisation based on erythritol fermentation to establish a Yarrowia based biorefinery in which both the fermentation broth and separated cells are utilised in high-value products. An important parameter of erythritol fermentation is the adequate oxygen level, so the effect of it was investigated in baffled flasks and fermentor as well. Furthermore, we measured the cosmetic applicability of cell lysates and the efficiency of biodetergent produced during erythritol fermentation. The results were showed that Yarrowia divulagata NCAIM 1485 strain could produce relative high amount of erythritol (44.14±1 g/l) under adequate oxygen supply while its cell lysate could exert good skin moisturizing effect. In all cases, the collected samples had emulsification index above 50% of which stability was also high, above 50% even after 24 hours.Therefore, it can be considered as an effective bioemulsifier. In conclusion, the erythritol fermentation of Y. divulgata is suitable for complex utilisation in a biorefinery, since the fermentation broth can be used for isolation of sweetener and bioemulsifier, meanwhile the cells can be processed for cosmetic application as skin moisturizer.
Biology and Life Sciences, Biology and Biotechnology
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