Version 1
: Received: 1 August 2023 / Approved: 2 August 2023 / Online: 3 August 2023 (02:42:07 CEST)
How to cite:
Bhatiya, M.; Jain, S.M.; Das, A.; Pathak, S.; Ahmad, S.F.; Duttaroy, A.K.; Banerjee, A. An in-Depth Study on the Anti-Oxidant and Anti-Apoptotic Potentials of Quercetin in Human Colon Cancer Cell Lines. Preprints2023, 2023080214. https://doi.org/10.20944/preprints202308.0214.v1
Bhatiya, M.; Jain, S.M.; Das, A.; Pathak, S.; Ahmad, S.F.; Duttaroy, A.K.; Banerjee, A. An in-Depth Study on the Anti-Oxidant and Anti-Apoptotic Potentials of Quercetin in Human Colon Cancer Cell Lines. Preprints 2023, 2023080214. https://doi.org/10.20944/preprints202308.0214.v1
Bhatiya, M.; Jain, S.M.; Das, A.; Pathak, S.; Ahmad, S.F.; Duttaroy, A.K.; Banerjee, A. An in-Depth Study on the Anti-Oxidant and Anti-Apoptotic Potentials of Quercetin in Human Colon Cancer Cell Lines. Preprints2023, 2023080214. https://doi.org/10.20944/preprints202308.0214.v1
APA Style
Bhatiya, M., Jain, S.M., Das, A., Pathak, S., Ahmad, S.F., Duttaroy, A.K., & Banerjee, A. (2023). An in-Depth Study on the Anti-Oxidant and Anti-Apoptotic Potentials of Quercetin in Human Colon Cancer Cell Lines. Preprints. https://doi.org/10.20944/preprints202308.0214.v1
Chicago/Turabian Style
Bhatiya, M., Asim K Duttaroy and Antara Banerjee. 2023 "An in-Depth Study on the Anti-Oxidant and Anti-Apoptotic Potentials of Quercetin in Human Colon Cancer Cell Lines" Preprints. https://doi.org/10.20944/preprints202308.0214.v1
Abstract
(1) Background: Colon cancer is one of the leading causes of cancer morbidity and mortality globally. It is a multi-step process that involves genetic and epigenetic modifications leading to histological and morphological changes. Several complementary therapeutic options have been analyzed, shedding light on plant-based medication as a potential treatment for colon cancer. Flavonoids such as quercetin are known to have anti-cancer and anti-inflammatory properties. This in vitro study examines quercetin's anti-inflammatory, anti-apoptotic, anti-angiogenesis effects and antioxidant properties in colon cancer cells. (2) Methods: The antioxidant capacity of quercetin-treated cells was investigated using biochemical assays, and angiogenesis and cytokine levels were assessed using enzyme-linked immunosorbent assay (ELISA). The epigenetic modulation and differential expression of aging, apoptotic, and proliferation genes, and Histone deacetylases (HDACs) were also investigated. (3) Results: In this study, the quercetin-treated group significantly reduced the antioxidant enzymes, cytokines, and VEGF levels, altering the expression of epigenetic factors. Quercetin also induced significant senescence in colon cancer cells. Moreover, a considerable increase was observed in the apoptotic and hTERT genes. In contrast, a decrease in p53, proliferation genes, and HDACs was observed, providing a basis for the clinical use of quercetin in cancer treatment. (4) Conclusion: In vitro studies showed that quercetin treatment efficiently induces senescence and apoptosis in colon cancer cells. We also found that quercetin effectively modulated the expression of p53, Wnt1, CTNNB1, and HDACs, indicating that it could be used to treat colon cancer.
Keywords
quercetin; antioxidant; apoptotic; anti-inflammatory activity; colon cancer
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
*
