Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Identification and Fine Mapping of Osdsm3, a Drought-Sensitive Gene in Rice (Oryza sativa L.)

Chenwei Deng
,
Yingxin Zhang
,
Beifang Wang
,
Hong Wang
ORCID logo ,
Pao Xue
,
Yongrun Cao
,
Lianping Sun
ORCID logo ,
Shihua Cheng
,
Liyong Cao
* ,
Daibo Chen
*
Version 1 : Received: 31 July 2023 / Approved: 1 August 2023 / Online: 2 August 2023 (07:58:40 CEST)

A peer-reviewed article of this Preprint also exists.

Deng, C.; Zhang, Y.; Wang, B.; Wang, H.; Xue, P.; Cao, Y.; Sun, L.; Cheng, S.; Cao, L.; Chen, D. Identification and Fine Mapping of Osdsm3, a Drought-Sensitive Gene in Rice (Oryza sativa L.). Agronomy 2023, 13, 2241. Deng, C.; Zhang, Y.; Wang, B.; Wang, H.; Xue, P.; Cao, Y.; Sun, L.; Cheng, S.; Cao, L.; Chen, D. Identification and Fine Mapping of Osdsm3, a Drought-Sensitive Gene in Rice (Oryza sativa L.). Agronomy 2023, 13, 2241.

Abstract

Drought poses a significant constraint on rice production, and in this study, we have discovered a novel drought-sensitive mutant, designated as dsm3, arising from the progenies of indica rice variety Zhonghui8015 treated with EMS. Under drought stress conditions, dsm3 exhibited characteristic withered leaf tips, accompanied by increased levels of MDA and H2O2, reduced Pn, and decreased activity of POD and SOD. Genetic analysis revealed that the withered leaf tip phenotype was governed by a single recessive gene, designated as Osdsm3.To begin with, Osdsm3 was initially mapped to the short arm of chromosome 1 through a cross involving dsm3 and 02428. Subsequently, utilizing a population of 2591 F2 individuals, we narrowed down the location of Osdsm3 to a 78Kb interval, encompassing 13 open reading frames (ORFs). Sequencing analysis unveiled a mutation (1275G→A) in the exon of the candidate gene (LOC_Os01g10680), leading to premature translation termination. Moreover, a quantitative RT-PCR assay demonstrated high expression of OsDSM3 in the panicle and sheath, with a significant upregulation of drought stress-related genes under drought conditions. Phylogenetic analyses indicated that Osdsm3 shares evolutionary homology with UNE1, an intracellular transport protein found in Arabidopsis thaliana. Subcellular studies further confirmed that OsDSM3 resides in the cytoplasm. In conclusion, the forthcoming cloning of Osdsm3 holds promise for delving deeper into the molecular mechanisms governing rice drought resistance.

Keywords

rice (Oryza sativa L.); drought stress; Osdsm3; genetic analysis; fine mapping

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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