Version 1
: Received: 19 July 2023 / Approved: 20 July 2023 / Online: 20 July 2023 (05:19:02 CEST)
How to cite:
King, J.L.; Shrivastava, R.; Shah, P.D.; Maturavongsadit, P.; Benhabbour, S.R. Injectable pH and Thermo‐Responsive Hydrogel Scaffold with Enhanced Osteogenic Differentiation of Preosteoblasts for Bone Regeneration. Preprints2023, 2023071379. https://doi.org/10.20944/preprints202307.1379.v1
King, J.L.; Shrivastava, R.; Shah, P.D.; Maturavongsadit, P.; Benhabbour, S.R. Injectable pH and Thermo‐Responsive Hydrogel Scaffold with Enhanced Osteogenic Differentiation of Preosteoblasts for Bone Regeneration. Preprints 2023, 2023071379. https://doi.org/10.20944/preprints202307.1379.v1
King, J.L.; Shrivastava, R.; Shah, P.D.; Maturavongsadit, P.; Benhabbour, S.R. Injectable pH and Thermo‐Responsive Hydrogel Scaffold with Enhanced Osteogenic Differentiation of Preosteoblasts for Bone Regeneration. Preprints2023, 2023071379. https://doi.org/10.20944/preprints202307.1379.v1
APA Style
King, J.L., Shrivastava, R., Shah, P.D., Maturavongsadit, P., & Benhabbour, S.R. (2023). Injectable pH and Thermo‐Responsive Hydrogel Scaffold with Enhanced Osteogenic Differentiation of Preosteoblasts for Bone Regeneration. Preprints. https://doi.org/10.20944/preprints202307.1379.v1
Chicago/Turabian Style
King, J.L., Panita Maturavongsadit and S. Rahima Benhabbour. 2023 "Injectable pH and Thermo‐Responsive Hydrogel Scaffold with Enhanced Osteogenic Differentiation of Preosteoblasts for Bone Regeneration" Preprints. https://doi.org/10.20944/preprints202307.1379.v1
Abstract
Bone fractures are very common in the geriatric population and poses a great economic burden worldwide. While traditional methods for repairing bone defects have primarily been autografts, there are several drawbacks limiting its use. Bone graft substitutes have been used as alternative strategies to improve bone healing. However, there remains several impediments to achieving the desired healing outcomes. Injectable hydrogels have become attractive scaffold materials for bone regeneration given their high performance in filling irregularly sized bone defects, ability to encapsulate cells and bioactive molecules, and mimic the native ECM of bone. We investigated the use of an injectable chitosan-based hydrogel scaffold to promote differentiation of preosteoblasts in vitro. The hydrogels were characterized by evaluating cell homogeneity, cell viability, rheological and mechanical properties, and differentiation ability of preosteoblasts in hydrogel scaffolds. Cell-laden hydrogel scaffolds exhibited shear thinning behavior and the ability to maintain shape fidelity after injection. The CNC-CS hydrogels exhibited higher mechanical strength and significantly upregulated the osteogenic activity and differentiation of preosteoblasts as shown by ALP activity assays and histological analysis of hydrogel scaffolds. These results suggest that this injectable hydrogel is suitable for cell survival, can promote osteogenic differentiation of preosteoblasts, and structurally support new bone growth.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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