Nam, D.E.; Cha, M.J.; Kim, Y.D.; Awasthi, M.; Do, Y.; Kong, S.-G.; Chung, K.W. Microsatellite Dataset for Cultivar Discrimination in Spring Orchid (Cymbidium goeringii). Genes2023, 14, 1610.
Nam, D.E.; Cha, M.J.; Kim, Y.D.; Awasthi, M.; Do, Y.; Kong, S.-G.; Chung, K.W. Microsatellite Dataset for Cultivar Discrimination in Spring Orchid (Cymbidium goeringii). Genes 2023, 14, 1610.
Nam, D.E.; Cha, M.J.; Kim, Y.D.; Awasthi, M.; Do, Y.; Kong, S.-G.; Chung, K.W. Microsatellite Dataset for Cultivar Discrimination in Spring Orchid (Cymbidium goeringii). Genes2023, 14, 1610.
Nam, D.E.; Cha, M.J.; Kim, Y.D.; Awasthi, M.; Do, Y.; Kong, S.-G.; Chung, K.W. Microsatellite Dataset for Cultivar Discrimination in Spring Orchid (Cymbidium goeringii). Genes 2023, 14, 1610.
Abstract
Cymbidium goeringii, locally known as the spring orchid in Korea, is one of the most important and popular horticultural species in the family Orchidaceae. C. goeringii cultivars were originated from plants with rare phenotypes in wild mountains where pine trees commonly grow. This study aimed to determine the cultivar-specific combined genotypes (CGs) of short sequence repeats (SSRs) by analyzing multiple samples per cultivar of C. goeringii. In this study, we collected more than 4,000 samples from 67 cultivars and determined the genotypes of 12 SSRs. Based on the most frequent combined genotypes (CG1s), the average observed allele number and combined matching probability were 11.8 per marker and 3.118×10-11, respectively. Frequencies of the CG1 in 50 cultivars (n ≥ 10) ranged from 40.9% to 100.0%, with an average of 70.1%. Assuming that individuals with the CG1 are genuine in the corresponding cultivars, approximately 30% of C. goeringii on the farms and markets may be not genuine. The dendrogram of the phylogenetic tree and principal coordinate analysis largely divided the cultivars into three groups according to their countries of origin; however, the genetic distances were not distant among the cultivars. In conclusion, this dataset of C. goeringii cultivar-specific SSR profiles could be used for ecogenetic studies and forensic authentication. This study suggests that genetic authentication should be introduced for the sale of expensive C. goeringii cultivars. We believe that this study will help establish a genetic method for the forensic authentication of C. goeringii cultivars.
Keywords
goeringii; cultivar authentication; microsatellite; simple sequence repeats (SSRs); spring orchid
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
