Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Redox Modulatory Potential of Methanol Extract of Pentaclethra Macrophylla Stem Bark

Petra Obioma Nnamani
* ORCID logo ,
Edith Obioma Diovu
,
Peter Ikechukwu
ORCID logo ,
Akachukwu M Onwuka
ORCID logo ,
Obiora Emmanuel Abonyi
,
Obinna Chibueze Ibezim
,
Birgitta Loretz
ORCID logo ,
Mariia Nesterkina
,
Claus- Michael Lehr
Version 1 : Received: 11 July 2023 / Approved: 11 July 2023 / Online: 12 July 2023 (08:56:04 CEST)

How to cite: Nnamani, P.O.; Diovu, E.O.; Ikechukwu, P.; Onwuka, A.M.; Abonyi, O.E.; Ibezim, O.C.; Loretz, B.; Nesterkina, M.; Lehr, C.M. Redox Modulatory Potential of Methanol Extract of Pentaclethra Macrophylla Stem Bark. Preprints 2023, 2023070776. https://doi.org/10.20944/preprints202307.0776.v1 Nnamani, P.O.; Diovu, E.O.; Ikechukwu, P.; Onwuka, A.M.; Abonyi, O.E.; Ibezim, O.C.; Loretz, B.; Nesterkina, M.; Lehr, C.M. Redox Modulatory Potential of Methanol Extract of Pentaclethra Macrophylla Stem Bark. Preprints 2023, 2023070776. https://doi.org/10.20944/preprints202307.0776.v1

Abstract

Purpose: Oxidative stress is responsible for over seventy five diseases and other harmful cytological challenges including cancer, type 2 diabetes mellitus, lipid peroxidation, distortion of cell membrane integrity, shortening of telomerase among others. Antioxidant potency of any plant is a very positive remedy to the debilitating effects of prooxidants and oxidants. The study was aimed at investigating the antioxidant potency of methanol extract of Pentaclethra macrophylla stem bark- in vitro and in vivo. Methods: Different phytochemicals were determined using standard methods. Nitric oxide, 1, 1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity, total antioxidant capacity and ferric reducing/anti-radical power were used to investigate the in vitro antioxidant activity. Catalase assay, superoxide dismutase, malondialdehyde and glutathione peroxidase were used to determine the in vivo antioxidant efficacy using six groups of five rats. Results: The result showed various quantities of phytochemicals, including antioxidant phytochemicals-flavonoids, tannins and total phenolics. The in vitro assay showed that 250 μg/ml of extract had the highest percentage inhibition of DPPH radical (93.93%). The 125 μg/ml had the highest scavenging activity of nitric oxide radical (49.47%) while 250μg/ml showed the least with 41.73%. Other methods showed various degrees of activity. The in vivo antioxidant showed that the principal mechanism of antioxidant activity is by modulating the iron-dependent catalase activity. Conclusion: Pentaclethra macrophylla has both in vitro and in vivo antioxidant activities and therefore is useful in ameliorating the debilitating effects of oxidants.

Keywords

Pentaclethera macrophylla stem bark; Methanol extract; Bergenin; Acute toxicity; Antioxidant; DPPH.

Subject

Medicine and Pharmacology, Pharmacy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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