Version 1
: Received: 4 July 2023 / Approved: 5 July 2023 / Online: 5 July 2023 (04:17:32 CEST)
How to cite:
Hormozi Jangi, S.R. A Comparative Study on the Kinetics Performances of Gold- and MnO2- Nanozymes. Preprints2023, 2023070258. https://doi.org/10.20944/preprints202307.0258.v1
Hormozi Jangi, S.R. A Comparative Study on the Kinetics Performances of Gold- and MnO2- Nanozymes. Preprints 2023, 2023070258. https://doi.org/10.20944/preprints202307.0258.v1
Hormozi Jangi, S.R. A Comparative Study on the Kinetics Performances of Gold- and MnO2- Nanozymes. Preprints2023, 2023070258. https://doi.org/10.20944/preprints202307.0258.v1
APA Style
Hormozi Jangi, S.R. (2023). A Comparative Study on the Kinetics Performances of Gold- and MnO<sub>2-</sub> Nanozymes. Preprints. https://doi.org/10.20944/preprints202307.0258.v1
Chicago/Turabian Style
Hormozi Jangi, S.R. 2023 "A Comparative Study on the Kinetics Performances of Gold- and MnO<sub>2-</sub> Nanozymes" Preprints. https://doi.org/10.20944/preprints202307.0258.v1
Abstract
Gold- and MnO2- nanozymes are well-known for their enzyme-like activity. In this regard, initially, gold- and MnO2- nanozymes were synthesized by simple and green methods. Afterward, the kinetic studies were performed using the Michaelis–Menten model for both gold- and MnO2- nanozymes. The kinetic parameters including Km and Vmax were calculated via the construction of the linear plot of Lineweaver–Burk for both nanozymes. The results showed a Vmax and Km of 185 nM sec-1 and 47 nM sec-1 for the gold- and MnO2- nanozymes, in order. The ratio of Vmax(gold)/Vmax(MnO2) was found to be about 4.0 which pointed that the catalytic efficiency of gold-nanozymes is 4.0-fold higher than the catalytic efficiency of MnO2- nanozymes. The Km value was found to be 0.72 mM and 1.6 mM for the as-prepared gold- and MnO2- nanozymes, respectively, and the Km of MnO2-nanozymes is 2.2-fold higher than that of gold nanozymes. Since the Km shows the affinity of substrate for binding to nanozyme active nodes (lower Km=higher affinity), it is consultable that the substrate affinity toward MnO2-nanozymes is 2.2-fold lower than that of the gold-nanozymes. Considering the above results, the as-prepared gold nanozymes are very stronger peroxidase-like mimics than the metal oxide MnO2-nanozymes.
Keywords
MnO2-nanozymes; Gold-nanozymes; Kinetic parameters; Comparative study
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
