preprints.org > chemistry and materials science > analytical chemistry > doi: 10.20944/preprints202306.2079.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 29 June 2023 / Approved: 29 June 2023 / Online: 29 June 2023 (09:47:55 CEST)

MnO₂ nanoparticles are considered nanozymes with intrinsic peroxidase-like activity and there are several reports on their application in the field of nanozyme-based sensing and detection, however, up to now, there is no report on the investigation of their biochemical and kinetics properties toward enzyme-mediated oxidations. Hence, in this work, the MnO₂ nanozymes were synthesized and characterized with different characterization methods. Thereafter, their biochemical properties including pH stability, thermal stability, and salt stability as well as their kinetics perfromances were evaluated toward n-electron irreversible oxidation of 3,3’-diaminobezedine. The results showed that the as-prepared nanozymes reveal their maximal enzyme-like activity over a wide pH range of 3.0-6.0 at a temperature of 23-25 ℃ toward oxidation of 3,3’-diaminobezedine. Besides, the salt stability studies exhibited that the as-mentioned nanozymes can save their maximal activity over a wide range of high salt concentrations over 3-7 M of NaCl. Moreover, the kinetics studies revealed a K_m as low as 1.6 mM and a V_max as high as 47 nM sec^-1 for the MnO₂ nanozymes toward irreversible oxidation of DAB to produce the brown-colored polyDAB.

MnO2 nanozyme; Brown-colored polyDAB; pH stability of nanozymes; Thermal stability of nanozymes; Kinetics of nanozymes

Chemistry and Materials Science, Analytical Chemistry

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