Version 1
: Received: 6 June 2023 / Approved: 27 June 2023 / Online: 27 June 2023 (12:36:13 CEST)
How to cite:
Kazemi, A.; Gharib, M.; Mohammadian Roshan, N.; Traz Jamshid, S.; Stögbauer, F.; Eslami, S.; Schüffler, P. Assessment of the Tumor-Stroma Ratio and Tumor Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation. Preprints2023, 2023061905. https://doi.org/10.20944/preprints202306.1905.v1
Kazemi, A.; Gharib, M.; Mohammadian Roshan, N.; Traz Jamshid, S.; Stögbauer, F.; Eslami, S.; Schüffler, P. Assessment of the Tumor-Stroma Ratio and Tumor Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation. Preprints 2023, 2023061905. https://doi.org/10.20944/preprints202306.1905.v1
Kazemi, A.; Gharib, M.; Mohammadian Roshan, N.; Traz Jamshid, S.; Stögbauer, F.; Eslami, S.; Schüffler, P. Assessment of the Tumor-Stroma Ratio and Tumor Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation. Preprints2023, 2023061905. https://doi.org/10.20944/preprints202306.1905.v1
APA Style
Kazemi, A., Gharib, M., Mohammadian Roshan, N., Traz Jamshid, S., Stögbauer, F., Eslami, S., & Schüffler, P. (2023). <strong>Assessment of the Tumor-Stroma Ratio and Tumor Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation</strong>. Preprints. https://doi.org/10.20944/preprints202306.1905.v1
Chicago/Turabian Style
Kazemi, A., Saeid Eslami and Peter Schüffler. 2023 "<strong>Assessment of the Tumor-Stroma Ratio and Tumor Infiltrating Lymphocytes in Colorectal Cancer: Inter-Observer Agreement Evaluation</strong>" Preprints. https://doi.org/10.20944/preprints202306.1905.v1
Abstract
Background: To implement the new marker in clinical practice, reliability assessment, validation, and standardization of utilization must be applied. This study evaluated the reliability of TILs and TSR assessment through conventional microscopy by comparing observers’ estimations. Methods: Intratumoral and tumor-front stromal TILs, and TSR were assessed by three pathologists using 86 HE slides CRC. TSR and TILs were categorized using one and four different proposed cut-off systems, respectively and agreement was assessed using the intraclass coefficient (ICC) and Cohen’s kappa statistics. Pairwise evaluation of agreement was performed using the Fliess Kappa statistic and the concordance rate and it was visualized by Bland-Altman plots. Results: For the evaluation of intratumoral stromal TILs, ICC of 0.505 (95% CI:0.35-0.64) was obtained, Kappa values were in the range of 0.21 to 0.38, and concordance rates in the range of 0.61 to 0.72. For the evaluation of tumor-front TILs, ICC was 0.52 (95% CI:0.32 - 0.67), the overall kappa value ranged from 0.24 to 0.30, and the concordance rate ranged from 0.66 to 0.72. For estimating the TSR, the ICC was 0.48 (95% CI:0.35 - 0.60), the kappa value was 0.49 and the concordance rate was 0.76. Conclusion: The agreement between pathologists in estimating these markers corresponds to poor to the moderate agreement, however, implementing immune scores in daily practice requires more concentration in inter-observer agreements.
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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