Chen, D.; Lei, S.; Sun, S.; Chen, Y.; Wang, J.; Gao, C.; Xie, X. Comparative Transcriptome Profiling of Skeletal Muscle From Fujian White Rabbit (Oryctolagus cuniculus) at Different Growth Stages Using RNA-Seq. Preprints2023, 2023060379. https://doi.org/10.20944/preprints202306.0379.v1
APA Style
Chen, D., Lei, S., Sun, S., Chen, Y., Wang, J., Gao, C., & Xie, X. (2023). Comparative Transcriptome Profiling of Skeletal Muscle From Fujian White Rabbit (Oryctolagus cuniculus) at Different Growth Stages Using RNA-Seq. Preprints. https://doi.org/10.20944/preprints202306.0379.v1
Chicago/Turabian Style
Chen, D., ChengFang Gao and XiPing Xie. 2023 "Comparative Transcriptome Profiling of Skeletal Muscle From Fujian White Rabbit (Oryctolagus cuniculus) at Different Growth Stages Using RNA-Seq" Preprints. https://doi.org/10.20944/preprints202306.0379.v1
Abstract
In China, the demand for rabbit meat is also increasing, and the production for rabbit meat is important. However, the underlying mechanisms of regulate skeletal muscle growth and development in rabbits remain unclear. The purpose of this study was to identify candidate genes related to skeletal muscle growth in rabbits and explore their potential regulatory mechanisms. RNA-seq technique was used to compare the differences of skeletal muscle transcriptome in Fujian white rabbits at different developmental stages (day 20 and 26 of embryos and birth 1, 30 and 60-day-olds) with a total of 25 individual selected. A total of 9737 DEGs that were acquired from the five groups were annotated into three ontologies of the GO database: biological process (BP), cell component (CC), and molecular function (MF). For KEGG analysis, there are 8249 genes were enriched in 1148 pathways, of which 67 pathways were significantly enriched, mainly in Hypertrophic cardiomyopathy (HCM), the PPAR signaling pathway, MAPK signaling pathway. Among all comparison groups, SE was the most abundant AS event. Real-time PCR verified that the expression patterns of differential genes were consistent with the transcriptome sequencing results. These results will provide a molecular regulation mechanism of muscle growth and development in Fujian white rabbits, and should serve an important theoretical basis for improving meat performance and growth rate of Chinese local meat rabbit breeds.
Keywords
skeletal muscle; transcriptome; gene expression; Fujian white rabbit; growth stage
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
