Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evaluating the Effect of Shelf-Storage, Daylight, and Air Oxygen on the Peroxidase-like Activity of Unmodified Silver Nanoparticles

Version 1 : Received: 28 May 2023 / Approved: 30 May 2023 / Online: 30 May 2023 (10:33:49 CEST)

A peer-reviewed article of this Preprint also exists.

Hormozi Jangi, S. R., & Dehghani, Z. (2023). Kinetics and biochemical characterization of silver nanozymes and investigating impact of storage conditions on their activity and shelf-life. Chemical Research and Nanomaterials, 1(4), 25-33. Hormozi Jangi, S. R., & Dehghani, Z. (2023). Kinetics and biochemical characterization of silver nanozymes and investigating impact of storage conditions on their activity and shelf-life. Chemical Research and Nanomaterials, 1(4), 25-33.

Abstract

In this contribution, unmodified silver nanoparticles were synthesized and characterized for their size and morphological properties. Thereafter, their peroxidase-like activity as the common catalytic property of silver nanoparticles was investigated by catalyzing the oxidation of 3,3’,5,5’-tetramethyl-benzidine (TMB) as peroxidase substrate, exhibiting, a specific activity as high as 5.4 µM min-1 for the as-prepared unmodified silver nanoparticles. The stability of the catalytic activity of the as-prepared nanozymes was also checked upon their storage at ambient temperature within 7 days at different storage conditions. The results revealed that the peroxidase-like activity of unmodified silver nanoparticles was approximately retained at about 75%, and 63% after 7 days exposing daylight and air oxygen, in order. The shelf-life (storage stability) of the as-prepared nanozymes was also investigated at usual storage conditions (i.e., 4 ℃ under dark), revealed that the nanozymes saved their activity about 96% of their initial activity after 10 days of storage at 4 ℃ under dark conditions.

Keywords

unmodified silver nanoparticles; peroxidase-like activity; air oxygen; daylight; shelf-life

Subject

Chemistry and Materials Science, Analytical Chemistry

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