Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Conferring High Iaa Productivity on Low-Iaa-Producing Organisms With PonAAS2, an Aromatic Aldehyde Synthase of a Galling Sawfly, and Identification of Its Inhibitor

Version 1 : Received: 29 May 2023 / Approved: 30 May 2023 / Online: 30 May 2023 (07:58:03 CEST)

A peer-reviewed article of this Preprint also exists.

Hiura, T.; Yoshida, H.; Miyata, U.; Asami, T.; Suzuki, Y. Conferring High IAA Productivity on Low-IAA-Producing Organisms with PonAAS2, an Aromatic Aldehyde Synthase of a Galling Sawfly, and Identification of Its Inhibitor. Insects 2023, 14, 598. Hiura, T.; Yoshida, H.; Miyata, U.; Asami, T.; Suzuki, Y. Conferring High IAA Productivity on Low-IAA-Producing Organisms with PonAAS2, an Aromatic Aldehyde Synthase of a Galling Sawfly, and Identification of Its Inhibitor. Insects 2023, 14, 598.

Abstract

Gall-inducing insects often contain high concentrations of phytohormones, such as auxin and cytokinin, which are suggested to be involved in gall induction, but no conclusive evidence has been obtained. There are two possible approaches to investigating the importance of phytohormones in gall induction: demonstrating either that high phytohormone productivity can induce gall-inducing ability in non-gall-inducing insects, or that gall-inducing ability is inhibited when phytohormone productivity in galling insects is suppressed. In this study, we show that the overexpression of PonAAS2, which encodes an aromatic aldehyde synthase (AAS) responsible for the rate-limiting step in indoleacetic acid (IAA) biosynthesis in a galling sawfly (Pontania sp.) that contains high levels of endogenous IAA, conferred high IAA productivity on Caenorhabditis elegans, as the model system. This result strongly suggests that PonAAS2 can also confer high IAA productivity on low-IAA-producing insects. We also successfully identified an inhibitor of PonAAS2 in a chemical library. This highly selective inhibitor showed stronger inhibitory activity against AAS than against aromatic amino acid decarboxylase, which belongs to the same superfamily as AAS. We also confirm that this inhibitor clearly inhibited IAA productivity in the high-IAA-producing C. elegans engineered here.

Keywords

phytohormone; auxin; insect gall; aromatic aldehyde synthase; inhibitor

Subject

Biology and Life Sciences, Insect Science

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