Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Phenolic Composition and Wound Healing Potential Assessment of Moroccan Henna (Lawsonia inermis) Aqueous Extracts

Soukaina El Massoudi
ORCID logo ,
Abdellah Zinedine
,
João Miguel Rocha
* ORCID logo ,
Meryem Benidir
,
Ilham Najjari
,
Lahsen El Ghadraoui
,
Meryem Benjelloun
,
Faouzi Errachidi
Version 1 : Received: 19 May 2023 / Approved: 22 May 2023 / Online: 22 May 2023 (05:30:59 CEST)

A peer-reviewed article of this Preprint also exists.

El Massoudi, S.; Zinedine, A.; Rocha, J.M.; Benidir, M.; Najjari, I.; El Ghadraoui, L.; Benjelloun, M.; Errachidi, F. Phenolic Composition and Wound Healing Potential Assessment of Moroccan Henna (Lawsonia inermis) Aqueous Extracts. Cosmetics 2023, 10, 92. El Massoudi, S.; Zinedine, A.; Rocha, J.M.; Benidir, M.; Najjari, I.; El Ghadraoui, L.; Benjelloun, M.; Errachidi, F. Phenolic Composition and Wound Healing Potential Assessment of Moroccan Henna (Lawsonia inermis) Aqueous Extracts. Cosmetics 2023, 10, 92.

Abstract

The present study aims at valorizing Moroccan Henna (Lawsonia inermis) by developing healing formulations for cosmetic and therapeutic uses. For such a goal, the plant was collected from three locations in Southeastern Morocco (Alnif, Tafraoute Sidi Ali and Tazarine). Phytochemical anal-yses of aqueous extracts of Henna leaves was performed by determining phenolic compounds contents, flavonoids and tannins in the extracts. Then, specific formulations were prepared using aqueous extracts of L. inermis to assess their in vivo wound healing potential in Swiss Albino mice as animal model. Results disclosed that phenolic compound contents (13.48%), flavonoids (9.25%) and tannins (2.57%) are higher in Henna leaf extracts from Alnif, while Tazarine Henna aqueous extract was found to be richer in saponins (0.32%). Exclusion chromatographic analysis on Se-phadex G50 gel corroborates the obtained results and shows that Lawsone levels (Henna coloring agent) are higher in Henna collected from Alnif. Aqueous Henna leaf extracts, at a dose of 10% in petroleum jelly, have been tested for their ability to heal induced burns in mice. Healing moni-toring, carried out with Henna extracts on mice batches and those of two control batches (Mice batch treated with petroleum jelly alone and batch treated with petroleum jelly containing 1% flamazine), showed a great reduction in burnt surface with an accentuated contraction percentage (CP) and complete re-epithelialization duration (CRD) at 21 days in the three studied Henna-based formulations. These findings suggest the interest of potential development of Henna-based for-mulations, as source of phenolic compounds, for further dermatological, cosmetic and therapeutic applications.

Keywords

Lawsonia inermis; Henna; leaves; phytochemical; wound healing activity; Morocco

Subject

Medicine and Pharmacology, Pharmacy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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