Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Açaí (Euterpe oleracea Mart.) Seed Oil Exerts a Cytotoxic Role Over Colorectal Cancer Cells: Insights of Annexin A2 Regulation and Molecular Modeling

Marcos Antonio Custódio Neto Da Silva
,
Josiane Weber Tessmann
,
Kátia Regina Assunção Borges
,
Laís Araújo Souza Wolff
,
Fernanda D. Botelho
ORCID logo ,
Leandro Alegria Vieira
,
Jose A Morgado-Diaz
ORCID logo ,
Tanos C. C. França
,
Maria do Carmo Lacerda Barbosa
,
Maria do Desterro Soares Brandão Nascimento
ORCID logo ,
Murilo R. Rocha
* ORCID logo ,
João Ernesto de Carvalho
*
Version 1 : Received: 17 May 2023 / Approved: 18 May 2023 / Online: 18 May 2023 (14:41:35 CEST)

A peer-reviewed article of this Preprint also exists.

da Silva, M.A.C.N.; Tessmann, J.W.; Borges, K.R.A.; Wolff, L.A.S.; Botelho, F.D.; Vieira, L.A.; Morgado-Diaz, J.A.; Franca, T.C.C.; Barbosa, M.C.L.; Nascimento, M.D.S.B.; Rocha, M.R.; de Carvalho, J.E. Açaí (Euterpe oleracea Mart.) Seed Oil Exerts a Cytotoxic Role over Colorectal Cancer Cells: Insights of Annexin A2 Regulation and Molecular Modeling. Metabolites 2023, 13, 789. da Silva, M.A.C.N.; Tessmann, J.W.; Borges, K.R.A.; Wolff, L.A.S.; Botelho, F.D.; Vieira, L.A.; Morgado-Diaz, J.A.; Franca, T.C.C.; Barbosa, M.C.L.; Nascimento, M.D.S.B.; Rocha, M.R.; de Carvalho, J.E. Açaí (Euterpe oleracea Mart.) Seed Oil Exerts a Cytotoxic Role over Colorectal Cancer Cells: Insights of Annexin A2 Regulation and Molecular Modeling. Metabolites 2023, 13, 789.

Abstract

Açaí, Euterpe oleracea Mart. is a native plant from the Amazonian and is rich in several phyto-chemicals with anti-tumor activities. The aim was to analyze the effects of açaí seed oil on colo-rectal adenocarcinoma (ADC) cells. In vitro analyses were performed on CACO-2, HCT-116, and HT-29 cell lines. The strains were treated with açaí seed oil for 24, 48, and 72 h, and cell viability, death, and morphology were analyzed. Molecular docking was performed to evaluate the inter-action between the major compounds in açaí seed oil and Annexin A2. The viability assay showed the cytotoxic effect of the oil in colorectal adenocarcinoma cells. Acai seed oil induced increased apoptosis in CACO-2 and HCT-116 cells and interfered with the cell cycle. Western blotting showed increased expression of LC3-B, suggestive of autophagy, and annexin A2, an apoptosis regulatory protein. Molecular docking confirmed the interaction of major fatty acids with annexin A2, suggesting a role of açaí seed oil in modulating annexin A2 expression in these cancer cell lines. Our results suggest the antitumor potential of açaí seed oil in colorectal adeno-carcinoma cells and contribute to the development of an active drug from a known natural product.

Keywords

Euterpe oleracea Mart; Polyphenols; Apoptosis; Autophagy; Annexin A2

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.