Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Transforming Growth Factor-Beta 1 Induces Neutrophil Apoptosis in Colorectal Cancer Liver Metastases

Miran Rada
* ORCID logo ,
Migmar Tsamchoe
,
Jessica Bloom
,
Diane H. Kim
,
Stephanie Petrillo
,
Anthoula Lazaris
ORCID logo ,
Peter Metrakos
*
Version 1 : Received: 5 May 2023 / Approved: 6 May 2023 / Online: 6 May 2023 (08:51:29 CEST)

How to cite: Rada, M.; Tsamchoe, M.; Bloom, J.; Kim, D.H.; Petrillo, S.; Lazaris, A.; Metrakos, P. Transforming Growth Factor-Beta 1 Induces Neutrophil Apoptosis in Colorectal Cancer Liver Metastases. Preprints 2023, 2023050396. https://doi.org/10.20944/preprints202305.0396.v1 Rada, M.; Tsamchoe, M.; Bloom, J.; Kim, D.H.; Petrillo, S.; Lazaris, A.; Metrakos, P. Transforming Growth Factor-Beta 1 Induces Neutrophil Apoptosis in Colorectal Cancer Liver Metastases. Preprints 2023, 2023050396. https://doi.org/10.20944/preprints202305.0396.v1

Abstract

Vessel co-option correlates with resistance against anti-angiogenic agents and chemotherapy in colorectal cancer liver metastasis (CRCLM). We previously identified higher intensity of neutrophils in the tumour microenvironment of vessel co-opting CRCLM lesions compared to their angiogenic counterparts. Herein, we demonstrated that over 50% of the neutrophils in vessel co-opting lesions are expressing pro-apoptotic markers including cleaved caspase-3 and poly (ADP-ribose) polymerase-1 (PARP-1). Our previous publications suggested upregulation of transforming growth factor-beta (TGFβ1) in the microenvironment of vessel co-option CRCLM. Therefore, we examined the effect of TGFβ1 on the expression of cleaved caspase-3 and PARP-1 in neutrophils in vitro. Significantly, we noticed the upregulation of pro-apoptotic markers upon exposure to TGFβ1. This finding might pave the way to determine the role of neutrophils in developing vessel co-option in CRCLM in the future.

Keywords

CRCLM; vessel co-option; angiogenesis; neutrophil; TGFβ1; apoptosis

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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