Version 1
: Received: 5 May 2023 / Approved: 6 May 2023 / Online: 6 May 2023 (08:51:29 CEST)
How to cite:
Rada, M.; Tsamchoe, M.; Bloom, J.; Kim, D. H.; Petrillo, S.; Lazaris, A.; Metrakos, P. Transforming Growth Factor-Beta 1 Induces Neutrophil Apoptosis in Colorectal Cancer Liver Metastases. Preprints2023, 2023050396. https://doi.org/10.20944/preprints202305.0396.v1
Rada, M.; Tsamchoe, M.; Bloom, J.; Kim, D. H.; Petrillo, S.; Lazaris, A.; Metrakos, P. Transforming Growth Factor-Beta 1 Induces Neutrophil Apoptosis in Colorectal Cancer Liver Metastases. Preprints 2023, 2023050396. https://doi.org/10.20944/preprints202305.0396.v1
Rada, M.; Tsamchoe, M.; Bloom, J.; Kim, D. H.; Petrillo, S.; Lazaris, A.; Metrakos, P. Transforming Growth Factor-Beta 1 Induces Neutrophil Apoptosis in Colorectal Cancer Liver Metastases. Preprints2023, 2023050396. https://doi.org/10.20944/preprints202305.0396.v1
APA Style
Rada, M., Tsamchoe, M., Bloom, J., Kim, D. H., Petrillo, S., Lazaris, A., & Metrakos, P. (2023). Transforming Growth Factor-Beta 1 Induces Neutrophil Apoptosis in Colorectal Cancer Liver Metastases. Preprints. https://doi.org/10.20944/preprints202305.0396.v1
Chicago/Turabian Style
Rada, M., Anthoula Lazaris and Peter Metrakos. 2023 "Transforming Growth Factor-Beta 1 Induces Neutrophil Apoptosis in Colorectal Cancer Liver Metastases" Preprints. https://doi.org/10.20944/preprints202305.0396.v1
Abstract
Vessel co-option correlates with resistance against anti-angiogenic agents and chemotherapy in colorectal cancer liver metastasis (CRCLM). We previously identified higher intensity of neutrophils in the tumour microenvironment of vessel co-opting CRCLM lesions compared to their angiogenic counterparts. Herein, we demonstrated that over 50% of the neutrophils in vessel co-opting lesions are expressing pro-apoptotic markers including cleaved caspase-3 and poly (ADP-ribose) polymerase-1 (PARP-1). Our previous publications suggested upregulation of transforming growth factor-beta (TGFβ1) in the microenvironment of vessel co-option CRCLM. Therefore, we examined the effect of TGFβ1 on the expression of cleaved caspase-3 and PARP-1 in neutrophils in vitro. Significantly, we noticed the upregulation of pro-apoptotic markers upon exposure to TGFβ1. This finding might pave the way to determine the role of neutrophils in developing vessel co-option in CRCLM in the future.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
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