Pan, S.-C.; Lai, C.-H.; Vu, V.-T.; Vu, C.-A.; Huang, C.-J.; Cheng, C.-M.; Chen, W.-Y. Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study. Int. J. Mol. Sci.2023, 24, 9822.
Pan, S.-C.; Lai, C.-H.; Vu, V.-T.; Vu, C.-A.; Huang, C.-J.; Cheng, C.-M.; Chen, W.-Y. Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study. Int. J. Mol. Sci. 2023, 24, 9822.
Pan, S.-C.; Lai, C.-H.; Vu, V.-T.; Vu, C.-A.; Huang, C.-J.; Cheng, C.-M.; Chen, W.-Y. Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study. Int. J. Mol. Sci.2023, 24, 9822.
Pan, S.-C.; Lai, C.-H.; Vu, V.-T.; Vu, C.-A.; Huang, C.-J.; Cheng, C.-M.; Chen, W.-Y. Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study. Int. J. Mol. Sci. 2023, 24, 9822.
Abstract
Emerging evidence has shown that microRNAs play pivotal roles in wound healing. MicroRNA-21 (miR-21) was previously found to upregulate in order to fulfill an anti-inflammation role for wounds. Exosomal miRNAs have been identified and explored as essential markers for diagnostic medicine. However, the role of exosomal miR-21 in wounds has yet to be well studied. In order to facilitate the early management of poorly healing wounds, we developed an easy-to-use, rapid, paper-based microfluidic-exosomal miR-21 extraction device to determine wound prognosis in a timely manner. We isolated and then quantitatively examined exosomal miR-21 in wound fluids from normal tissues, acute and chronic wounds. Eight improving wounds displayed lower levels of exosomal miR-21 expression after wound debridement. However, four instances of increased exosomal miR-21 expression levels were notably associated with patients with poor healing wounds despite aggressive wound debridement, indicating a predictive role of tissue exosomal miR-21 for wound outcome. Paper-based nucleic acid extraction device provides a rapid and user-friendly approach for evaluating exosomal miR-21 in wound fluids as a means of monitoring wounds. Our data suggest that tissue exosomal miR-21 is a reliable marker for determining current wound status.
Keywords
P-ELISA; exosome; microRNA-21; wound healing
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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