MacDonald, A.B. Invasion of Chronic Lymphocytic Leukemia ( CLL/SLL) Bloodstream Tumor Cells by Borrelia spirochetes. Preprints2023, 2023050157. https://doi.org/10.20944/preprints202305.0157.v1
APA Style
MacDonald, A.B. (2023). Invasion of Chronic Lymphocytic Leukemia ( CLL/SLL) Bloodstream Tumor Cells by B<em>orrelia spirochetes</em>. Preprints. https://doi.org/10.20944/preprints202305.0157.v1
Chicago/Turabian Style
MacDonald, A.B. 2023 "Invasion of Chronic Lymphocytic Leukemia ( CLL/SLL) Bloodstream Tumor Cells by B<em>orrelia spirochetes</em>" Preprints. https://doi.org/10.20944/preprints202305.0157.v1
Abstract
A sixty-six-year-old woman with a strong family history of breast cancer noted gradual onset of axillary lymph node enlargement in 2019. Axillary lymph node biopsies disclosed Lymph node involvement by Chronic Lymphocytic Leukemia (CLL/SLL). Immunophenotype analysis of nodal tissue and of bloodstream leukemic cells in immunohistochemistry and flow cytometry and next generation molecular diagnostics confirmed CLL/SLL. Replicate absolute peripheral blood lymphocyte counts in excess of 5.0 x 10e3/microliter confirmed the numerical threshold for diagnosis of Chronic Lymphocytic Leukemia in the bloodstream. FISH studies disclosed an immunoglobulin “mutation negative” CLL/SLL genotype without a light chain restriction pattern. No translocations or germline mutations were present. Borrelia spirochetes in the bloodstream were detected with simultaneous high spirochetemia of both borrelia miyamotoi and borrelia burgdorferi. FISH hybridization of leukemic lymphocytes disclosed extensive borrelia invasion of the cytoplasm and of the nucleus compartments of CLL/SLL tumor cells. Retrospective studies of the patient’s archival stored blood smears obtained three years prior in 2016 at age 63 disclosed no evidence of CLL/SLL but confirmed asymptomatic bloodstream borrelia miyamotoi and borrelia burgdorferi borrelia infection. Lyme serology had been reported as negative in 2016. Additionally, retrospective FISH DNA hybridizations focused on preleukemic bloodstream Lymphocytes disclosed rare borrelia spirochetal adherence to benign blood lymphocytes in the year 2016 in the preleukemic blood smears. This case report is the very first to describe precursor chronic asymptomatic Lyme borreliosis and Miyamotoi borrelia bloodstream infections three years prior to the diagnosis of Rai Stage 1 Chronic Lymphocytic leukemia.
Medicine and Pharmacology, Oncology and Oncogenics
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