Version 1
: Received: 26 April 2023 / Approved: 29 April 2023 / Online: 29 April 2023 (08:02:54 CEST)
How to cite:
Wang, G.; Zhang, Z.; Yin, C.; Lv, Z. The Putative Neurotransmitter Transporter Blot Regulates F-actin Organization in Drosophila. Preprints2023, 2023041227. https://doi.org/10.20944/preprints202304.1227.v1
Wang, G.; Zhang, Z.; Yin, C.; Lv, Z. The Putative Neurotransmitter Transporter Blot Regulates F-actin Organization in Drosophila. Preprints 2023, 2023041227. https://doi.org/10.20944/preprints202304.1227.v1
Wang, G.; Zhang, Z.; Yin, C.; Lv, Z. The Putative Neurotransmitter Transporter Blot Regulates F-actin Organization in Drosophila. Preprints2023, 2023041227. https://doi.org/10.20944/preprints202304.1227.v1
APA Style
Wang, G., Zhang, Z., Yin, C., & Lv, Z. (2023). The Putative Neurotransmitter Transporter Blot Regulates F-actin Organization in Drosophila. Preprints. https://doi.org/10.20944/preprints202304.1227.v1
Chicago/Turabian Style
Wang, G., Chuanhao Yin and Zhiyi Lv. 2023 "The Putative Neurotransmitter Transporter Blot Regulates F-actin Organization in Drosophila" Preprints. https://doi.org/10.20944/preprints202304.1227.v1
Abstract
Solute carrier (SLC) proteins transport a wide range of substrates across biological membranes and more than 400 proteins are considered as SLCs based on its sequence. Despite it has important potential applications in drug development, the SLC superfamily is still understudied. Drosophila blot, an orphan SLC protein, regulates F-actin organization in the syncytial blastoderm and the Malpighian tubules. However, the molecular mechanism was unknown. In this study, we found Blot was localized at the furrow canal, the front of the invaginating membrane during cellularization process. Blot is required for maintaining proper morphology of furrow canal and cellularization process. By affinity purification and mass spectrometry we identified RhoGEF ELMO/DOCK complex was required for Blot-mediated F-actin organization and cellularization. Further analysis revealed that ELMO localization depends on Blot. Taken together, we propose Blot plays a role in F-actin organization as an upstream factor of ELMO by recruiting ELMO to the target membrane compartments.
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
