Version 1
: Received: 25 April 2023 / Approved: 26 April 2023 / Online: 26 April 2023 (05:15:36 CEST)
How to cite:
Taher, S.M.; Abdo, J.M.; Merza, M.A. Whole Genome Sequence Analysis of SARS-COV-2 Isolated from COVID-19 Patients in Duhok Province, Iraq. Preprints2023, 2023040961. https://doi.org/10.20944/preprints202304.0961.v1
Taher, S.M.; Abdo, J.M.; Merza, M.A. Whole Genome Sequence Analysis of SARS-COV-2 Isolated from COVID-19 Patients in Duhok Province, Iraq. Preprints 2023, 2023040961. https://doi.org/10.20944/preprints202304.0961.v1
Taher, S.M.; Abdo, J.M.; Merza, M.A. Whole Genome Sequence Analysis of SARS-COV-2 Isolated from COVID-19 Patients in Duhok Province, Iraq. Preprints2023, 2023040961. https://doi.org/10.20944/preprints202304.0961.v1
APA Style
Taher, S.M., Abdo, J.M., & Merza, M.A. (2023). Whole Genome Sequence Analysis of SARS-COV-2 Isolated from COVID-19 Patients in Duhok Province, Iraq. Preprints. https://doi.org/10.20944/preprints202304.0961.v1
Chicago/Turabian Style
Taher, S.M., Jassim Mohammed Abdo and Muayad Aghali Merza. 2023 "Whole Genome Sequence Analysis of SARS-COV-2 Isolated from COVID-19 Patients in Duhok Province, Iraq" Preprints. https://doi.org/10.20944/preprints202304.0961.v1
Abstract
Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in people in the city of Wuhan at the end of December 2019, and it has since spread to all continents. Global interest has been focused on discovering a treatment and developing a successful coronavirus vaccine as a result of the emergence of this new coronavirus. In this research, we sequenced the whole genome of the SARS-COV-2 virus that were isolated from 40 patients in Duhok, Iraq. 95 different mutations were identified in our isolation when such the whole genome sequence of the SARS-COV-2 isolated from the city of Wuhan, China (Accession number: NC 045512.2) was matched to the virus's sequence using Sequencing technology (Illumina , USA ) and Assembly method (iVar 1.3.1). Sequence analysis revealed that 38 mutations were found at spike glycoprotein (S), 30 of which were found in the ORF1b , 11 mutations were found in ORF1b ,7,3,2,1 mutations were found in ( N,M,ORF6 ,ORF9 /E ) genes which had been linked to structural changes at various places. The conclusion that the cases in Iraq were of different origins of infections and had a close relationship with the isolates from different country and state were further confirmed by phylogenetic analysis and transmission. The whole genome sequence of the SARS-CoV-2 , which were identified from the Iraqi Kurdistan region, and reported as a first study in Kurdistan region.
Keywords
COVID-19 patient; whole Genome sequence; Of SARS-COV-2; phylogenetic analysis and Transmission supported
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
