Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Failure of Micractinium simplicissimum Phosphate Resilience upon Abrupt Re-Feeding of its Phosphorus-Starved Cultures

Version 1 : Received: 20 April 2023 / Approved: 21 April 2023 / Online: 21 April 2023 (08:29:53 CEST)

A peer-reviewed article of this Preprint also exists.

Lobakova, E.; Gorelova, O.; Selyakh, I.; Semenova, L.; Scherbakov, P.; Vasilieva, S.; Zaytsev, P.; Shibzukhova, K.; Chivkunova, O.; Baulina, O.; Solovchenko, A. Failure of Micractinium simplicissimum Phosphate Resilience upon Abrupt Re-Feeding of Its Phosphorus-Starved Cultures. Int. J. Mol. Sci. 2023, 24, 8484. Lobakova, E.; Gorelova, O.; Selyakh, I.; Semenova, L.; Scherbakov, P.; Vasilieva, S.; Zaytsev, P.; Shibzukhova, K.; Chivkunova, O.; Baulina, O.; Solovchenko, A. Failure of Micractinium simplicissimum Phosphate Resilience upon Abrupt Re-Feeding of Its Phosphorus-Starved Cultures. Int. J. Mol. Sci. 2023, 24, 8484.

Abstract

Microalgae are naturally adapted to fluctuating availability of phosphorus (P) being capable to opportunistically uptake large amounts of inorganic phosphate (Pi) and safely store it in the cell as polyphosphate. Hence, many microalgal species are remarkably resilient to high concentrations of external Pi. Here we report on an exception from this pattern comprised by a failure of the high Pi-resilience in a strain Micractinium simplicissimum IPPAS C-2056 normally coping with a very high Pi concentrations. This phenomenon occurred after abrupt re-supplementation of Pi to the M. simplicissimum culture pre-starved of P. This was the case even if Pi was re-supplemented in a concentration far below the level toxic to the P-sufficient culture. We hypothesize that this effect can be mediated by a rapid formation of the potentially toxic short-chain polyphosphate following the mass influx of Pi into the P-starved cell. A possible reason for this is that the preceding P starvation impairs the capacity of the cell of converting the newly absorbed Pi into a “safe” storage form of long-chain polyphosphate. We believe that findings of this study can help to avoid sudden culture crashes, they are also of potential significance for development of algae-based technologies for efficient bioremoval of P from P-rich waste streams.

Keywords

Micractinium; inorganic phosphate; polyphosphate; phosphorus toxicity

Subject

Biology and Life Sciences, Biology and Biotechnology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.