Version 1
: Received: 13 April 2023 / Approved: 14 April 2023 / Online: 14 April 2023 (05:02:28 CEST)
How to cite:
Shevade, K. Modeling Neurodegenerative/Neurodevelopmental Disorders using Human-Derived Pluripotent Stem Cells. Preprints2023, 2023040352. https://doi.org/10.20944/preprints202304.0352.v1
Shevade, K. Modeling Neurodegenerative/Neurodevelopmental Disorders using Human-Derived Pluripotent Stem Cells. Preprints 2023, 2023040352. https://doi.org/10.20944/preprints202304.0352.v1
Shevade, K. Modeling Neurodegenerative/Neurodevelopmental Disorders using Human-Derived Pluripotent Stem Cells. Preprints2023, 2023040352. https://doi.org/10.20944/preprints202304.0352.v1
APA Style
Shevade, K. (2023). Modeling Neurodegenerative/Neurodevelopmental Disorders using Human-Derived Pluripotent Stem Cells. Preprints. https://doi.org/10.20944/preprints202304.0352.v1
Chicago/Turabian Style
Shevade, K. 2023 "Modeling Neurodegenerative/Neurodevelopmental Disorders using Human-Derived Pluripotent Stem Cells" Preprints. https://doi.org/10.20944/preprints202304.0352.v1
Abstract
Human induced pluripotent stem cells (hiPSCs) have two characteristic abilities 1) self-renewal and 2) differentiation into all cell types in the human body. It is this ability of iPSCs to differentiate into any desired cell type that makes them amenable to disease modeling. iPSCs can further be engineered to carry a disease-specific mutation or be derived from patients to phenocopy the disease. To model neurodegenerative/neurodevelopmental disorders iPSCs are differentiated into neurons. This chapter describes protocols for two different approaches to generate neurons from iPSCs, first using dual SMAD inhibition to generate neural progenitor cells which are then differentiated into neurons, and second using single transcription factor (NGN2) over-expression to drive differentiation of iPSCs directly into neurons.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
