Alam, Md.M.; Akash, S.R. In Vitro Pharmacological Activities of Methanol Extract of Acmella Oleracea Leaves: A Variety Grown in Dhaka, Bangladesh. Indian Journal of Microbiology Research 2023, 10, 43–49, doi:10.18231/j.ijmr.2023.008.
Alam, Md.M.; Akash, S.R. In Vitro Pharmacological Activities of Methanol Extract of Acmella Oleracea Leaves: A Variety Grown in Dhaka, Bangladesh. Indian Journal of Microbiology Research 2023, 10, 43–49, doi:10.18231/j.ijmr.2023.008.
Alam, Md.M.; Akash, S.R. In Vitro Pharmacological Activities of Methanol Extract of Acmella Oleracea Leaves: A Variety Grown in Dhaka, Bangladesh. Indian Journal of Microbiology Research 2023, 10, 43–49, doi:10.18231/j.ijmr.2023.008.
Alam, Md.M.; Akash, S.R. In Vitro Pharmacological Activities of Methanol Extract of Acmella Oleracea Leaves: A Variety Grown in Dhaka, Bangladesh. Indian Journal of Microbiology Research 2023, 10, 43–49, doi:10.18231/j.ijmr.2023.008.
Abstract
Acemella oleracea is known as toothache plant belongs to the family of Asteraceae. It is treated as a medicinal remedy like tuberculosis, illness, cough, rheumatism, and illness. This study was concentrated on the antioxidant, cytotoxic, antimicrobial & thrombolytic activities of methanol extract of Acmella oleracea leaves using in vitro model. The antioxidant activity was estimated as trolox equivalent antioxidant capacity utilizing the DPPH and reducing power tests. The plant extract was tested for its cytotoxic action using a brine shrimp lethality bioassay, thrombolytic activity using clot disruption, and antibacterial activity using a disc diffusion assay technique against four distinct gram-positive and gram-negative bacteria. IC50 value of standard ascorbic acid for DPPH was 7.8µg/ml and the IC50 of the methanol extract of Acmella oleracea leaves was 198.34µg/ml that was moderate effect at all compare with ascorbic acid. Thrombolytic assay of Streptokinase as a positive control showed 88.49% where the extract shows 18.69% lytic activity shows the assay. In this study, the sample of LC50 results of cytotoxicity assay was 1.431 µg/mL which can be treated as less activity. Moreover, the extracts showed low to moderate antibacterial activity against both gram-negative and gram-positive bacterial stains (zone of inhibition-10-26 mm). Vibrio Mimicus bacteria stains exhibited the highest level of activity, with a range of 23 for the diameter of the growth inhibition zone. The current review shows leaf extracts of Acmella oleracea may be used as a source of antioxidant and thrombolytic activity, as well as a significant source of antibacterial and anticancer substances. Further research is required to evaluate in-vivo the pharmacological activity of Acmella oleracea leaves in order to identify the essential metabolites and potential mechanisms.
Keywords
Acmella oleracea; Anti-oxidant; In vitro; Anti-bacterial; Methanol extract
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
