PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in Monoraphidium sp. for Pharmacological and Nutraceutical Applications
Version 1
: Received: 21 March 2023 / Approved: 22 March 2023 / Online: 22 March 2023 (01:49:20 CET)
How to cite:
Yadav, K.; Kumar, S.; Nikalje, G.C.; Rai, M.P. Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in Monoraphidium sp. for Pharmacological and Nutraceutical Applications. Preprints2023, 2023030382. https://doi.org/10.20944/preprints202303.0382.v1
Yadav, K.; Kumar, S.; Nikalje, G.C.; Rai, M.P. Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in Monoraphidium sp. for Pharmacological and Nutraceutical Applications. Preprints 2023, 2023030382. https://doi.org/10.20944/preprints202303.0382.v1
Yadav, K.; Kumar, S.; Nikalje, G.C.; Rai, M.P. Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in Monoraphidium sp. for Pharmacological and Nutraceutical Applications. Preprints2023, 2023030382. https://doi.org/10.20944/preprints202303.0382.v1
APA Style
Yadav, K., Kumar, S., Nikalje, G.C., & Rai, M.P. (2023). Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications. Preprints. https://doi.org/10.20944/preprints202303.0382.v1
Chicago/Turabian Style
Yadav, K., Ganesh Chandrakant Nikalje and Monika Prakash Rai. 2023 "Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications" Preprints. https://doi.org/10.20944/preprints202303.0382.v1
Abstract
Carotenoids are essential pigments that play a vital role in the photosynthetic process of algae and are important for human health and nutrition. Despite their potential value, algal carotenoids are being neglected by biofuel industries. Recent research is aimed at producing microalgal lipids and carotenoids simultaneously under abiotic stresses. In this study, the effect of different abiotic stress factors such as Nitrogen (N), Potassium (K), Phosphorous (P), Sulphur (S), light intensity, and photoperiod on biomass, lipid, and carotenoids synthesis in Monoraphidium sp. (MP) were explored utilizing a statistical method. In addition, 2, 7-dichlorofluorescein diacetate (DCF-DA) mediated spectrofluorimetry was employed to detect the ele-vation in ROS level. The results revealed that variations in nutrients and light con-ditions are the key factors in the up-regulation of biomolecules lipids and carote-noids in the algae. The carotenoids profiling was done by Ultra Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectroscopy (UPLC-Q-ToF-MS). Among consisting carotenoids violaxanthin, astaxanthin, and beta-carotene were enhanced by 1.3 folds, 1.19 folds, 1.08 folds, while lutein was declined by 1.32 folds under stressed condition compared to control. The identified carotenoids are rec-ognized for their importance in the pharmaceutical and nutraceutical industries, highlighting the significant role of MP in these sectors.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
