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Multiple Pesticide Resistance in Rust-Red Fluor Beetle (Tribolium castaneum, Herbst 1797) from Northern Nigeria is Probably Driven by Metabolic Mechanisms
Mukhtar, M.M.; Mustapha, M.A.; Aliyu, M.; Ibrahim, S.S. Multiple Pesticide Resistance in Rust-Red Flour Beetle (Tribolium castaneum, Herbst 1797) from Northern Nigeria Is Probably Driven by Metabolic Mechanisms. Agrochemicals2023, 2, 170-180.
Mukhtar, M.M.; Mustapha, M.A.; Aliyu, M.; Ibrahim, S.S. Multiple Pesticide Resistance in Rust-Red Flour Beetle (Tribolium castaneum, Herbst 1797) from Northern Nigeria Is Probably Driven by Metabolic Mechanisms. Agrochemicals 2023, 2, 170-180.
Mukhtar, M.M.; Mustapha, M.A.; Aliyu, M.; Ibrahim, S.S. Multiple Pesticide Resistance in Rust-Red Flour Beetle (Tribolium castaneum, Herbst 1797) from Northern Nigeria Is Probably Driven by Metabolic Mechanisms. Agrochemicals2023, 2, 170-180.
Mukhtar, M.M.; Mustapha, M.A.; Aliyu, M.; Ibrahim, S.S. Multiple Pesticide Resistance in Rust-Red Flour Beetle (Tribolium castaneum, Herbst 1797) from Northern Nigeria Is Probably Driven by Metabolic Mechanisms. Agrochemicals 2023, 2, 170-180.
Abstract
Information about molecular mechanism of pesticide resistance in the rust-red flour beetle, a major pest destroying grains and flour across Nigeria is grossly lacking, hindering evidence-based con-trol. Here, we identified to species level three populations of the red-flour beetle from Kano, Ni-geria, as Tribolium castaneum, and investigated the mechanism driving their insecticide resistance. IRAC susceptibility bioassays established high cypermethrin resistance, with LC50s of 4.35-5.46mg/ml in the three populations, NNFM, R/Zaki and Yankaba. High DDT and malathion resistance was observed in NNFM, with LC50s of 15.32- and 3.71mg/ml, respectively. High sus-ceptibility was observed towards dichlorvos in all three populations with LC50s of 0.17-0.35mg/ml, respectively. Synergist bioassay with piperonylbutoxide significantly restored cypermethrin sus-ceptibility, with mortality increasing almost threefold, from 24.8% obtained from 1.25mg/ml of cypermethrin, to 63.3% in synergised group (p=0.013), suggesting a preeminent role of P450s. The two major knockdown resistance (kdr) mutations, T929I and L1014F in the IIS4 and IIS6 fragments of voltage-gated sodium channel were not detected in both cypermethrin-alive and cyperme-thrin-dead beetles, suggesting lesser role of target-site insensitivity. These findings highlight the need to explore alternative control tools for this pest and/or explore incorporation of synergists, like piperonylbutoxide as additional chemistries into pesticides formulations to improve their ef-ficacy.
Keywords
red flour beetle; Tribolium castaneum; pesticides; insecticides; resistance, metabolic
Subject
Biology and Life Sciences, Insect Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
