Shao, Y.; Tian, R.; Duan, J.; Wang, M.; Cao, J.; Cao, Z.; Li, G.; Jin, F.; Abd El-Aty, A.M.; She, Y. A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water. Sensors2023, 23, 649.
Shao, Y.; Tian, R.; Duan, J.; Wang, M.; Cao, J.; Cao, Z.; Li, G.; Jin, F.; Abd El-Aty, A.M.; She, Y. A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water. Sensors 2023, 23, 649.
Shao, Y.; Tian, R.; Duan, J.; Wang, M.; Cao, J.; Cao, Z.; Li, G.; Jin, F.; Abd El-Aty, A.M.; She, Y. A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water. Sensors2023, 23, 649.
Shao, Y.; Tian, R.; Duan, J.; Wang, M.; Cao, J.; Cao, Z.; Li, G.; Jin, F.; Abd El-Aty, A.M.; She, Y. A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water. Sensors 2023, 23, 649.
Abstract
Glyphosate (GLYP) is a broad-spectrum, non-selective, organic phosphine post emergence herbicide registered for use on many food and non-food field. Herein, we developed a biosensor (Mbs@dsDNA) based on carboxylated modified magnetic beads incubated with NH2-polyA and then hybridized with polyT-glyphosate aptamer and complementary DNA. Afterward, a quantitative detection method based on qPCR was established. When the glyphosate aptamer on Mbs@dsDNA specifically recognized glyphosate, a complementary DNA is released and then enters the qPCR signal amplification process. The linear range of the method was 0.1-5 μg/mL, and the detection limit was set at 0.1 μg/mL. The recoveries in tap water were ranged from 103.4 ~ 104.9%, and the relative standard deviations (RSDs) were < 1%. The aptamer proposed in this study has a good potential for recognizing glyphosate. The detection method combined with qPCR might have a good application prospect in detecting and supervising other pesticide residues.
Keywords
Glyphosate; Aptamer; qPCR; SYBR Green I; sensor
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
