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A Decade of CRISPR-Cas Genome Editing in C. elegans
Version 1
: Received: 11 November 2022 / Approved: 17 November 2022 / Online: 17 November 2022 (02:22:43 CET)
A peer-reviewed article of this Preprint also exists.
Kim, H.-M.; Hong, Y.; Chen, J. A Decade of CRISPR-Cas Gnome Editing in C. elegans. Int. J. Mol. Sci. 2022, 23, 15863. Kim, H.-M.; Hong, Y.; Chen, J. A Decade of CRISPR-Cas Gnome Editing in C. elegans. Int. J. Mol. Sci. 2022, 23, 15863.
Abstract
CRISPR-Cas allows us to introduce desired genome editing, including mutations, epitopes, and deletions with unprecedented efficiency. The development of CRISPR-Cas has progressed to such an extent that it is now applicable in various fields with the help of model organisms. C. elegans is one of the pioneering animals in which numerous CRISPR-Cas strategies have been rapidly es-tablished over the past decade. Ironically, the emergence of numerous methods makes the right choice of method difficult. Choosing an appropriate selection or screening approach is the first step in planning a genome modification. This report summarizes the key features and applications of CRISPR-Cas methods using C. elegans and illustrates key strategies. Our overview of significant advances in CRISPR-Cas will help readers to understand current advances in genome editing and navigate various methods of CRISPR-Cas genome editing.
Keywords
Genome editing 4; C. elegans 5; Genome engineering; CRISPR; CRISPR-Cas
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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