Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

BcAMT1;5 Mediates Nitrogen Influxes in Flowering Chinese Cabbage and Improves Plant Growth when Overexpressed in Arabidopsis

Version 1 : Received: 15 November 2022 / Approved: 16 November 2022 / Online: 16 November 2022 (10:20:07 CET)

How to cite: Zhu, Y.; Zhong, L.; Huang, X.; Su, W.; Liu, H.; Sun, G.; Song, S.; Chen, R. BcAMT1;5 Mediates Nitrogen Influxes in Flowering Chinese Cabbage and Improves Plant Growth when Overexpressed in Arabidopsis. Preprints 2022, 2022110305. https://doi.org/10.20944/preprints202211.0305.v1 Zhu, Y.; Zhong, L.; Huang, X.; Su, W.; Liu, H.; Sun, G.; Song, S.; Chen, R. BcAMT1;5 Mediates Nitrogen Influxes in Flowering Chinese Cabbage and Improves Plant Growth when Overexpressed in Arabidopsis. Preprints 2022, 2022110305. https://doi.org/10.20944/preprints202211.0305.v1

Abstract

Nitrogen (N) is a major limiting factor for plant growth and vegetable production. Understanding the regulatory mechanisms of N uptake, transport, and assimilation is key to improving nitrogen use efficiency in plants. Ammonium transporters (AMTs) play an important role in plant N metabolism. In this study, we isolated an important AMT1 subfamily member (BcAMT1;5) with a highly conserved signatural AMT1 subfamily motif from flowering Chinese cabbage. Based on functional complementation in yeast mutant 31019b and overexpression of BcAMT1;5 in Arabidopsis, BcAMT1;5 is a functional ammonium transporter. Tissue expression analysis showed that BcAMT1;5 was mainly expressed in roots and showed multiple N regime transcript patterns to respond to varying nutritional conditions. This was up-regulated by N-deficiency and down-regulated by supplying NH4+. The glucuronidase (GUS) activities of BcAMT1;5pro::GUS showed a similar change in response to different N conditions. Overexpression of BcAMT1;5 accelerated the growth of transgenic seedlings, increased NH4+ net influxes, and enhanced the content and accumulation of NH4+ and NO3- at low N concentrations. Additionally, it increased the transcript levels of N assimilation-related genes in shoots. These results indicate that the transcriptional regulation of BcAMT1;5 in flowering Chinese cabbage may participate in N uptake and assimilation under various N conditions.

Keywords

ammonium transport; flowering Chinese cabbage; NH4+; NO3-; ion influx; overexpression

Subject

Biology and Life Sciences, Horticulture

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