Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster

Marco Antonio Cabrera González; Ana Alice Maia Gonçalves; Jennifer Ottino; Jaqueline Costa Leite; Lucilene Aparecida Resende; Otoni Alves Melo-Júnior; Patrícia Silveira; Mariana Santos Cardoso; Ricardo T. Fujiwara; Lilian Bueno; Renato Lima Santos; Tatiane Furtado de Carvalho; GIANI MARTINS GARCIA; Paulo Ricardo de Oliveira Paes; Alexsandro Sobreira Galdino; Miguel Angel Chávez-Fumagalli; Marília Martins; Denise da Silveira-Lemos; Olindo Assis Martins-Filho; Walderez Ornelas Dutra; Vanessa Mosqueira; Rodolfo Cordeiro Giunchetti

doi:10.20944/preprints202211.0275.v1

A peer-reviewed article of this Preprint also exists.

González, M.A.C.; Gonçalves, A.A.M.; Ottino, J.; Leite, J.C.; Resende, L.A.; Melo-Júnior, O.A.; Silveira, P.; Cardoso, M.S.; Fujiwara, R.T.; Bueno, L.L.; Santos, R.L.; Carvalho, T.F.; Garcia, G.M.; Paes, P.R.O.; Galdino, A.S.; Chávez-Fumagalli, M.A.; Melo, M.M.; Silveira-Lemos, D.; Martins-Filho, O.A.; Dutra, W.O.; Mosqueira, V.C.F.; Giunchetti, R.C. Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster. Vaccines 2023, 11, 111. González, M.A.C.; Gonçalves, A.A.M.; Ottino, J.; Leite, J.C.; Resende, L.A.; Melo-Júnior, O.A.; Silveira, P.; Cardoso, M.S.; Fujiwara, R.T.; Bueno, L.L.; Santos, R.L.; Carvalho, T.F.; Garcia, G.M.; Paes, P.R.O.; Galdino, A.S.; Chávez-Fumagalli, M.A.; Melo, M.M.; Silveira-Lemos, D.; Martins-Filho, O.A.; Dutra, W.O.; Mosqueira, V.C.F.; Giunchetti, R.C. Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster. Vaccines 2023, 11, 111.

Abstract

Visceral leishmaniasis (VL)is a fatal disease caused by the protozoa Leishmania infantum for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of L. infantum. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, [poly (D, L-lactic) acid (PLA) polymer] loading Leishmania amazonensis antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-Leishmania IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups showed an intense reduction in liver and spleen parasitic load by qPCR. The LAPSmP and LAPSmG vaccines showed exceptional results, indicating that they may be promising candidates as a VL vaccine.

Keywords

Subject

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Not displayed online.

Please leave your comment to this article below.

Mathematical equations can be typed in either LaTeX formats \\[ ... \\] or $$ ... $$, or MathML format <math> ... </math>. Try the LaTeX or MathML example.

Type equation:

Preview:

Copy equation into message below

Close menu

Please click a symbol to insert it into the message box below:

Please enter the link here:

Optionally, you can enter text that should appear as linked text:

Copy link into message below

Please enter or paste the URL to the image here (please only use links to jpg/jpeg, png and gif images):

Copy image into message below

Type author name or keywords to filter the list of references in this group (you can add a new citation under Bibliography):

No existing citations in Discussion Group

Wikify editor is a simple editor for wiki-style mark-up. It was written by MDPI for Sciforum in 2014. The rendering of the mark-up is based on Wiky.php with some tweaks. Rendering of mathematical equations is done with MathJax. Please send us a message for support or for reporting bugs.

Please declare any conflicts of interest you may have with this paper, financial or otherwise.

I do not have a conflict of interest

I would like to declare a conflict of interest

Display my name on the website

Captcha

Renew

I accept the following conditions:

Comments must follow the standards of professional discourse and should focus on the scientific content of the article. Insulting or offensive language, personal attacks and off-topic remarks will not be permitted. Comments must be written in English. Preprints reserves the right to remove comments without notice. Readers who post comments are obliged to declare any competing interests, financial or otherwise.

Vaccination with Formulation of Nanoparticles Loaded with Leishmania amazonensis Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster

Abstract

Keywords

Subject

Comments (0)